Immunofluorescence analysis of Plastin-L was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with L-Plastin (LPL4A.1) Mouse Monoclonal Antibody (MA5-11921) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant L-plastin protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||2 ug/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11921 targets Plastin-L in IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-11921 immunogen is human recombinant L-plastin protein.
L-plastin is a leukocyte specific actin bundling protein from fimbrin family of proteins. It has two actin-binding domains and two EF hand type calcium-binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L-plastin may play a role in regulating integrin mediated adhesion in leukocytes. L-plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosteronse in andrgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Fascin Rigidity and L-plastin Flexibility Cooperate in Cancer Cell Invadopodia and Filopodia.
MA5-11921 was used in immunocytochemistry to study cooperation in cancer cell invadopodia and filopodia by fascin rigidity and L-plastin flexibility
|Van Audenhove I,Denert M,Boucherie C,Pieters L,Cornelissen M,Gettemans J||The Journal of biological chemistry (291:9148)||2016|
L-plastin nanobodies perturb matrix degradation, podosome formation, stability and lifetime in THP-1 macrophages.
MA5-11921 was used in immunocytochemistry and western blot to study the role of L-plastin in THP-1 macrophage function using nanobodies directed against L-plastin
|De Clercq S,Boucherie C,Vandekerckhove J,Gettemans J,Guillabert A||PloS one (8:null)||2013|
Nanobody-induced perturbation of LFA-1/L-plastin phosphorylation impairs MTOC docking, immune synapse formation and T cell activation.
MA5-11921 was used in immunocytochemistry and western blot to study the disruption of the formation of immune synapses, MTOC docking and T-cell activation by single domain camelid-derived antibodies targeting L-plastin
|De Clercq S,Zwaenepoel O,Martens E,Vandekerckhove J,Guillabert A,Gettemans J||Cellular and molecular life sciences : CMLS (70:909)||2013|
L-plastin Ser5 phosphorylation in breast cancer cells and in vitro is mediated by RSK downstream of the ERK/MAPK pathway.
MA5-11921 was used in western blot to study signal transduction pathways that result in L-plastin Ser5 phosphorylation using 4 human breast cancer cell lines
|Lommel MJ,Trairatphisan P,Gäbler K,Laurini C,Muller A,Kaoma T,Vallar L,Sauter T,Schaffner-Reckinger E||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (30:1218)||2016|
Metastasis of prostate cancer and melanoma cells in a preclinical in vivo mouse model is enhanced by L-plastin expression and phosphorylation.
MA5-11921 was used in western blot to study the role of L-plastin phosphorylation in prostate cancer and melanoma metastsis
|Riplinger SM,Wabnitz GH,Kirchgessner H,Jahraus B,Lasitschka F,Schulte B,van der Pluijm G,van der Horst G,Hämmerling GJ,Nakchbandi I,Samstag Y||Molecular cancer (13:null)||2014|
Proteomic analysis of mononuclear cells of patients with minimal-change nephrotic syndrome of childhood.
MA5-11921 was used in western blot to study the proteome of mononuclear cells of patients with minimal-change nephrotic syndrome of childhood
|González E,Neuhaus T,Kemper MJ,Girardin E||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (24:149)||2009|
Antileukoproteinase: modulation of neutrophil function and therapeutic effects on anti-type II collagen antibody-induced arthritis.
MA5-11921 was used in western blot to study the therapeutic effect of antileukoproteinase an antibody transfer model of erosive polyarthritis
|Sehnert B,Cavcic A,Böhm B,Kalden JR,Nandakumar KS,Holmdahl R,Burkhardt H||Arthritis and rheumatism (50:2347)||2004|
|Not Applicable||Not Cited||
Analysis of the expression of biomarkers in urinary bladder cancer using a tissue microarray.
MA5-11921 was used in immunohistochemistry - paraffin section to characterize Akt, PTEN, Drg-1, Cx-26, and L-plastin expression and their relationship to the outcome of bladder cancer
|Harris LD,De La Cerda J,Tuziak T,Rosen D,Xiao L,Shen Y,Sabichi AL,Czerniak B,Grossman HB||Molecular carcinogenesis (47:678)||2008|
Phosphorylation of ectopically expressed L-plastin enhances invasiveness of human melanoma cells.
MA5-11921 was used in immunohistochemistry to investigate the effect of L-plastin phosphorylation on the invasiveness of human melanoma cells
|Klemke M,Rafael MT,Wabnitz GH,Weschenfelder T,Konstandin MH,Garbi N,Autschbach F,Hartschuh W,Samstag Y||International journal of cancer (120:2590)||2007|