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|Tested species reactivity||All|
|Host / Isotype||Not Applicable|
|Immunogen||Taq DNA Polymerase|
|Storage buffer||TBS, pH 8, with 1mM DTT, 0.1mM EDTA, 50% glycerol, 2mM Na2PO4|
|Tested Applications||Dilution *|
|Functional Assay (FN)||Assay dependent|
|Inhibition Assays (IA)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Platinum® Taq Antibody blocks Taq DNA polymerase activity at ambient temperatures but releases active polymerase after the denaturation step in PCR cycling at 94°C. By increasing the effectiveness of Taq DNA polymerase through use of this product, it is possible to reduce the optimization and handling of reaction components and improve PCR results. Platinum® Taq Antibody is supplied in a concentration sufficient to eliminate non-specific priming during amplification (5 U/µl). To prepare an antibody:polymerase complex, Platinum® Taq Antibody is mixed with an equal number of units of Taq DNA polymerase. The mixture is used at the diluted enzyme unit concentration with no other modifications to PCR reactions necessary. Other native, recombinant, or truncated forms of Taq DNA polymerase,4,5 including Elongase® Enzyme (Catalog Number 10480-010/-028), are also effectively inhibited.
One unit of Taq DNA polymerase incorporates 10 nmol of deoxyribonucleotide into DNA in 30 minutes at 74°C. One unit is defined as the amount of product required to inhibit one unit of Taq DNA polymerase.
PCR Functional Assay Specifications: PCR reactions containing 200 ng, 20 ng and 10 ng of input human genomic DNA yield 40 ng, 10 ng and 5 ng of an 800 bp target, respectively, when observed on an ethidium bromide stained gel.
In a radioactive nucleotide incorporation assay, Taq DNA polymerase activity has been successfully inhibited by 10965-010.
Platinum® Taq Antibody is a proprietary inhibitor which, when bound to Taq DNA polymerase, inactivates polymerase activity. This reagent provides an automatic “hot start” for Taq DNA polymerase in PCR. Hot starts are typically used in PCR to increase sensitivity, specificity, and yield while allowing assembly of reactions at ambient temperatures. The extra time, effort, and contamination risks associated with manual hot start procedures are addressed with the use of Platinum® Taq Antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Taq DNA polymerase