Immunofluorescence analysis of Podocalyxin (PODXL) was performed using 90% confluent log phase HEK 293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PODXL (3D3) Mouse Monoclonal Antibody (39-3800) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human podocalyxin fusion protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Podocalyxin promotes glioblastoma multiforme cell invasion and proliferation by inhibiting angiotensin-(1-7)/Mas signaling.
39-3800 was used in western blot to investigate the effect of crosstalk between PODX and Ang-(1-7)/Mas signaling in glioblastoma multiforme cells
|Liu B,Liu Y,Jiang Y||Oncology reports (33:2583)||2015|
Bmi1 essentially mediates podocalyxin-enhanced Cisplatin chemoresistance in oral tongue squamous cell carcinoma.
39-3800 was used in western blot to study the molecular basis for cisplatin chemoresistance in oral tongue squamous cell carcinoma
|Zhou Y,Zhang L,Pan H,Wang B,Yan F,Fang X,Munnee K,Tang Z||PloS one (10:null)||2015|
Podocalyxin promotes glioblastoma multiforme cell invasion and proliferation via ß-catenin signaling.
39-3800 was used in western blot to explored the impact of crosstalk between PODX and beta-cat signaling in glioblastoma multiform cells
|Liu Y,Yang L,Liu B,Jiang YG||PloS one (9:null)||2014|
Sperm¿associated antigen 9 promotes astrocytoma cell invasion through the upregulation of podocalyxin.
39-3800 was used in western blot to study the relationship between SPAG9 and PODXL in human astrocytoma invasion
|Jiang J,Liu Y,Fang W,Liu F||Molecular medicine reports (10:417)||2014|
Podocalyxin regulates astrocytoma cell invasion and survival against temozolomide.
39-3800 was used in western blot to test the effect of PODXL on astrocytoma cell invasion and survival against a chemotherapy agent.
|Wu H,Yang L,Liao D,Chen Y,Wang W,Fang J||Experimental and therapeutic medicine (5:1025)||2013|
PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression.
39-3800 was used in western blot to determine if PINCH1 translocates to the nucleus and regulates gene expression.
|Wang D,Li Y,Wu C,Liu Y||PloS one (6:null)||2011|
|Human||Not Cited||Enhanced podocalyxin expression alters the structure of podocyte basal surface.||Economou CG,Kitsiou PV,Tzinia AK,Panagopoulou E,Marinos E,Kershaw DB,Kerjaschki D,Tsilibary EC||Journal of cell science (117:3281)||2004|
|Human||Not Cited||Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin.||Schopperle WM,Kershaw DB,DeWolf WC||Biochemical and biophysical research communications (300:285)||2003|
A dimeric PINK1-containing complex on depolarized mitochondria stimulates Parkin recruitment.
39-3800 was used in immunohistochemistry and western blot to investigate the molecular mechanism that result in PINK1-mediated dissipation of the mitochondrial membrane potential.
|Okatsu K,Uno M,Koyano F,Go E,Kimura M,Oka T,Tanaka K,Matsuda N||The Journal of biological chemistry (288:36372)||2013|
|Mouse||Not Cited||Stimulatory effects of cardiotrophin 1 on atherosclerosis.||Konii H,Sato K,Kikuchi S,Okiyama H,Watanabe R,Hasegawa A,Yamamoto K,Itoh F,Hirano T,Watanabe T||Hypertension (Dallas, Tex. : 1979) (62:942)||2013|
|Human||1:80||Overexpression of the anti-adhesin podocalyxin is an independent predictor of breast cancer progression.||Somasiri A,Nielsen JS,Makretsov N,McCoy ML,Prentice L,Gilks CB,Chia SK,Gelmon KA,Kershaw DB,Huntsman DG,McNagny KM,Roskelley CD||Cancer research (64:5068)||2004|
||Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin.||Schopperle WM,Kershaw DB,DeWolf WC||Biochemical and biophysical research communications (300:285)||2003|