Western blot of Podocin/NPHS2 in (A) human kidney and (B) mouse kidney lysates was performed using 35ug protein in RIPA buffer. Lysates were probed with a Podocin/NPHS2 polyclonal antibody (Product# PA5-37904) at a dilution of 0.3ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human, Mouse, Pig|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-SPSKPVEPLNPKK, from the C-terminus of NPHS2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with cow based on sequence homology.
Podocin (NPHS2 protein) is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.