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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Syrian hamster / IgG|
|Immunogen||Murine thymic stromal cell lines|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:400|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:1000-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
This antibody does not react with human samples.
Podoplanin antibody (Clone 8.1.1) is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR3/FLT4, another marker for lymphatic endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.
MA5-16113 was used in flow cytometry to examine the role of lymph node stromal cells in immune responses
|Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE||eLife (3:null)||2014|
aggrus; glycoprotein 38; Gp38; PA2.26 antigen; podoplanin; RANDAM-2; RP23-348F1.2; T1a; T1alpha; transmembrane glycoprotein E11
Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; T1-alpha; T1a; T1alpha