Western blot analysis of podoplanin in lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with a Podoplanin monoclonal antibody (Product # MA5-16270) at 5 ug/ml. Goat anti-rat Ig HRP secondary antibody.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a, kappa|
|Immunogen||Synthetic peptide corresponding to amino acids 38-51 (EGGVAMPGAEDDVV) from the platelet-aggregation-stimulating (PLAG) domain of human podoplanin was used as immunogen.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 µg/10^5 cells|
|Western Blot (WB)||3-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Podoplanin, also known as gp38, is a 43kD type 1 transmembrane glycoprotein found on many tissues. Podoplanin appears to be differentially expressed by lymphatic endothelial cells but not blood vessel endothelial cells. Podoplanin has also been found to be expressed on a wide variety of tumors. It appears to be involved in lymphangioigenesis and promoting cell migration. Recently, there are indications of podoplanin presence on Th17 cells. In a murine EAE model for autoimmune disease it has been shown that Th17 cells migrate quickly to the CNS at EAE onset. Injection of antibody to podoplanin in mice also immunized with myelin antigen to induce EAE results in elevated numbers of Th17 cells in the CNS. Thus as in effecting migration of tumor cells, podoplanin may also promote migration of committed Th17 cells to sites of inflammation in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.