Western blot analysis of CD155 was performed by loading 25 ug of mouse stomach (Lane 1), U-937 (Lane 2) and Hela (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a CD155 monoclonal antibody (Product # MA5-13493) at a dilution of 1:20 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 45 kDa. This image was obtained using the unconjugated form of the antibody.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Western Blot (WB)||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13490 targets CD155 in FACS, WB and ICC/IF applications and shows reactivity with Human, Mouse and Non-human primate samples.
The MA5-13490 immunogen is heLa cells.
PVR is a member of Ig-superfamily with 3 Ig-domains in arrangement V-C-C. Four mRNAs are produced from single gene for PVR, (mapped to human chromosome band 19q13.1-13.2). Two of the mRNAs (called H20A and H20B or alpha and kappa,) encode cell surface molecules of about 43kDa and 45kDa polypeptide backbone, respectively. Two other splice variants, beta and gamma encode secreted molecules of about 44kDa. The cytoplasmic tails are short (aa 35-50) and are rich in serine phosphorylated residues. MW of PVR is.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of Necl-5 (CD155/PVR) reduces glioblastoma dispersal and decreases MMP-2 expression and activity.
MA5-13490 was used in immunocytochemistry to investigate the effect of Necl-5 blockade on glioblastoma progression and specific gene expression
|Enloe BM,Jay DG||Journal of neuro-oncology (102:225)||2011|
CD155/PVR enhances glioma cell dispersal by regulating adhesion signaling and focal adhesion dynamics.
MA5-13490 was used in immunocytochemistry to investigate the effect of CD155/PVR on glioma cell adhesion
|Sloan KE,Stewart JK,Treloar AF,Matthews RT,Jay DG||Cancer research (65:10930)||2005|
Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.
MA5-13490 was used in immunocytochemistry to study CD155-dependent endocytosis and retrograde axonal transport of polivirus-containg vessicles
|Ohka S,Matsuda N,Tohyama K,Oda T,Morikawa M,Kuge S,Nomoto A||Journal of virology (78:7186)||2004|
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
MA5-13490 was used in immunohistochemistry to investigate the effect of natural killer cells on Ewing sarcoma and its mechanism
|Verhoeven DH,de Hooge AS,Mooiman EC,Santos SJ,ten Dam MM,Gelderblom H,Melief CJ,Hogendoorn PC,Egeler RM,van Tol MJ,Schilham MW,Lankester AC||Molecular immunology (45:3917)||2008|
Identification of secreted CD155 isoforms.
MA5-13490 was used in western blot to study secreted human CD155 isoforms
|Baury B,Masson D,McDermott BM,Jarry A,Blottière HM,Blanchardie P,Laboisse CL,Lustenberger P,Racaniello VR,Denis MG||Biochemical and biophysical research communications (309:175)||2003|