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|Tested species reactivity||Chemical|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgM|
|Storage buffer||tissue culture supernatant|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
MA1-40133 detects Polymyxin B.
MA1-40133 has been successfully used in Western blot, and Immunoassay applications.
The MA1-40133 immunogen is Polymixin B.
The monoclonal antibody 45 reacts with Polymyxin B. The antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS.
The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody 45 enables the possibilities to study quantitatively the interaction of PMB and LPS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Polymyxins as novel and safe mucosal adjuvants to induce humoral immune responses in mice.
MA1-40133 was used in ELISA to study the use of polymyxin antibiotics as mucosal adjuvants during intranasal immunization in mice
|Yoshino N,Endo M,Kanno H,Matsukawa N,Tsutsumi R,Takeshita R,Sato S||PloS one (8:null)||2013|