|Tested species reactivity||Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from rat voltage gated potassium channel, Kv2.2-subunit conjugated to KLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for the ~125k voltage gated potassium channel, Kv 2.2 subunit.
A suggested positive control for this product is rat brain lysate.
Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pong , 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variation in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK) channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety on non-neuronal cell types including pancreatic islet , alveolar cells and cardiac myocytes (Hwang et al , 1993; Yan et al , 2004; Michaelevski et al , 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.