|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||4.5 ug/ml|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||0.75 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.75 ug/ml|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-751 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2.
MA1-751 has successfully been used immunofluorescence, immunocytochemistry, immunohistochemistry, Western blot , and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites.
The MA1-751 immunogen is a synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.
Familial Alzheimer and quote;s disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.
PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies.
MA1-751 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies
|Diehlmann A,Ida N,Weggen S,Grünberg J,Haass C,Masters CL,Bayer TA,Beyreuther K||Journal of neuroscience research (56:405)||1999|