Immunofluorescent analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) (Product# MA1-752) shows staining in A2058 melanoma cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 (Product# MA1-752) at a dilution of 1:20-1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues C S(313) K Y N A E S T E R E S Q D T V A E N D D G(334) of humen PS1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (IHC)||1:20 - 1:200|
|Western Blot (WB)||1:50 - 1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-752 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2.
MA1-752 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot, immunoprecipitation, and ELISA procedures. By Western blot, this antibody detects an ~18 kDa protein representing PS1 CT (C-terminus fragment) and the ~46 kDa full-length PS1 from transfected SH-SY5Y cells. In 4% paraformaldehyde fixed N2a cells, MA1-752 showed endogenous PS1 staining in the Golgi and cellular vesicles.
The MA1-752 immunogen is a synthetic peptide corresponding to residues C S(313) K Y N A E S T E R E S Q D T V A E N D D G(334) of humen PS1.
Familial Alzheimer and quote;s disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.
PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ß-Secretase 1's Targeting Reduces Hyperphosphorilated Tau, Implying Autophagy Actors in 3xTg-AD Mice.
MA1-752 was used in western blot to study reduction of hyperphosphorilated Tau by beta-secretase 1's targeting implying autophagy actors in 3xTg-AD mice
|Piedrahita D,Castro-Alvarez JF,Boudreau RL,Villegas-Lanau A,Kosik KS,Gallego-Gomez JC,Cardona-Gómez GP||Frontiers in cellular neuroscience (9:null)||2016|
Self-renewal and differentiation of reactive astrocyte-derived neural stem/progenitor cells isolated from the cortical peri-infarct area after stroke.
MA1-752 was used in western blot to study the self-renewal and differentiation of reactive astrocyte-derived neural stem/progenitor cells isolated after stroke
|Shimada IS,LeComte MD,Granger JC,Quinlan NJ,Spees JL||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:7926)||2012|
Direct interaction of Alzheimer's disease-related presenilin 1 with armadillo protein p0071.
MA1-752 was used in immunoprecipitation to investigate the interaction between presenilin 1 and armadillo protein p0071
|Stahl B,Diehlmann A,Südhof TC||The Journal of biological chemistry (274:9141)||1999|
Abrogation of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase activation.
MA1-752 was used in immunoprecipitation to investigate the impacts of caspase activation on heterodimeric presenilin 1 complex and the interaction between PS1 and beta-catenin
|Tesco G,Kim TW,Diehlmann A,Beyreuther K,Tanzi RE||The Journal of biological chemistry (273:33909)||1998|