Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of MKN45 (Lane 1). The blot was probed with Anti-Presinillin 1 Chicken Monoclonal Antibody (Product # PA1-1752, 1 ug/ml) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate (Product # A16054, 1:4000 dilution). Two isoforms of 53 and 42 kDa corresponding to Presinillin 1 was observed across the cell line tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE and reg ; 10% Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Mixture of synthetic peptides corresponding to residues K(311) N S K Y N A E S T E R(322) and E(342) A Q R D S H L G P H R(353) of human PS1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1752 detects presenilin 1 protein (PS1) from human, rat and mouse samples.
PA1-1752 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~66 kDa protein representing PS1.
The PA1-1752 immunogen is a synthetic peptides corresponding to residues K(311) N S K Y N A E S T E R(322) and E(342) A Q R D S H L G P H R(353) of human PS1.
Familial Alzheimer and quote;s disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.
PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.