Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 26) (Product# MA1-754) shows staining in A2058 Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product# MA1-754) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||4.5 ug/ml|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||0.75 ug/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA1-754 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.
MA1-754 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot and ELISA procedures. By Western blot, this antibody detects an ~20 kDa protein representing PS2 CT (C-terminal fragment) and the ~45 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed RAW cells, MA1-754 showed specific Golgi and ER labeling.
The MA1-754 immunogen is a synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.
Familial Alzheimer and quote;s disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.
PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Abrogation of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase activation.
MA1-754 was used in immunoprecipitation to investigate the impacts of caspase activation on heterodimeric presenilin 1 complex and the interaction between PS1 and beta-catenin
|Tesco G,Kim TW,Diehlmann A,Beyreuther K,Tanzi RE||The Journal of biological chemistry (273:33909)||1998|