Western blot analysis of Presenilin enhancer protein 2 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse intestine lysate using a Presenilin enhancer protein 2 polyclonal antibody (Product # PA5-23447) at 3 and 7 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Bovine, Dog, Horse, Human, Marsupial, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (2 NLERVSNEEKLNLCRKYY 19) of Presenilin enhancer protein 2 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||3-7 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Presenilin enhancer protein 2 (PEN-2) is a part of a gamma-secreted complex which catalyses the final cleavage of amyloid precursor protein to generate the toxic amyloid beta protein, the major component of plaque in the brains of Alzheimer disease patients. It is an essential subunit of the gamma-secretase complex, and an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). And probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.