|Tested species reactivity||Sheep|
|Published species reactivity||Rabbit, Bovine|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide corresponding to 146-R(154)R(171)-182 of ovine PrP|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the scrapie-associated protease-resistant isoform (PrPsc) of ovine prion protein (PrP). The prion protein is encoded by the PrP gene and is constitutively expressed as the normal cellular isoform PrPc by many cell types. Clone 2G11 specifically recognizes the R151-R159 sequence and does not recognize the non-pathogenic PrPc form.
Pre-treat samples with trypsin at 37°C for 5 minutes followed by heat-mediated antigen retrieval using 10mM citrate buffer (pH6.0), followed by treatment of tissue sections in 98% formic acid for 30 minutes for optimal results.
Transmissible spongiform encephalopathies (TSE) are neurodegenerative diseases in which the accumulation of the abnormal PrPsc isoform in tissues, of the lymphoid and central nervous system, is thought to be a key event. Studies suggest that PrPsc is the causative agent of transmissible spongiform encephalitis (TSE) but this has not been confirmed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Modulatory role of phosphoinositide 3-kinase in gastric acid secretion.
MA1-82828 was used in western blot to investigate the role of phosphoinositide 3-kinase in gastric acid secretion
|Mettler SE,Ghayouri S,Christensen GP,Forte JG||American journal of physiology. Gastrointestinal and liver physiology (293:G532)||2007|
High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells.
MA1-82828 was used in immunohistochemistry to show the subcellular localization of hydrogen/potassium-ATPase in gastric parietal cells
|Sawaguchi A,McDonald KL,Forte JG||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:77)||2004|
Functionally distinct pools of actin in secretory cells.
MA1-82828 was used in immunocytochemistry to investigate the importance of cytoskeleton in the secretion of HCl in stomach
|Ammar DA,Nguyen PN,Forte JG||American journal of physiology. Cell physiology (281:C407)||2001|
Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase.
MA1-82828 was used in immunocytochemistry to investigate the role of RAB11a N124I for stimulatory recruitment of the hydrogen-potassium-ATPase in gastric parietal cells
|Duman JG,Tyagarajan K,Kolsi MS,Moore HP,Forte JG||The American journal of physiology (277:C361)||1999|
Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor.
MA1-82828 was used in immunocytochemistry to study the role of receptor extracellular domains in protein trafficking
|Dintzis SM,Velculescu VE,Pfeffer SR||The Journal of biological chemistry (269:12159)||1994|