|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant fragment of human procalcitonin.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20289 detects procalcitonin in human samples.
MA1-20289 has been successfully used in ELISA and Western blot procedures.
The MA1-20289 immunogen is a recombinant fragment of human procalcitonin.
Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. It belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 a.a.r. from N-terminus. PCT is produced normally in C-cells of the thyroid glands. It undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.r), calcitonin (32 a.a.r.) and katacalcin (21 a.a.r.). It has been shown that the level of PCT in serum increases significantly during an infection of bacterial origin (Assicot M, et al). Today PCT is considered to be one of the earliest and most specific markers of sepsis. However, serveral studies revealed that elevated PCT level in human blood could be detected not only in cases of sepsis and infection, but also in cases of surgery, polytrauma, heat shock and cardiogenic shock (Meisner M. and Reinhart K). The importance of PCT measurements in combination of cTnT or cTnl during heart transplantation to predict and early graft failure has been proven (Potapo EV., et al).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.