Western blot analysis of Progerin in Hela cells expressing flag-tagged progerin using a Progerin monoclonal antibody (Product # MA5-18073) at various dilutions (lanes 1-3) compared to a control Lamin A/C antibody (lane 4) that recognizes Lamin A and C as well as eptopic progerin. Hela samples were boiled in Laemmli buffer, separated by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was blocked in 3% NFDM-PBST for 1 hr at room temp, incubated at 4°C in 0.5% NFDM-PBST, and detected using an HRP-conjugated Fc-specific anti-mouse secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 604-611 (GAQSPQNC) at the C terminus of Human Progerin|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody specifically recognizes Progerin (Lamin A delta50) and does not cross-react with endogenous prelamin A.
Western blot results show a band at approximately 70 kDa.
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.