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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||The region encoding amino acids 1-101 of the human progesterone receptor expressed in E. coli.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23267 detects Progesterone receptor in Human samples.
MA1-23267 has been successfully used in Western Blot procedures. The predicted molecular weight is 99 kDa. Suggested positive control for this product are MCF7 lysate and recombinant protein.
MA1-23267 immunogen corresponds to The region encoding amino acids 1-101 of the human progesterone receptor expressed in E. coli.
The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR cn positively or negatively regulated gene transcription through the reruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
NR3C3; nuclear receptor subfamily 3 group C member 3; PGR
NR3C3; PGR; PR