Immunohistochemistry was performed on normal deparaffinized Human uterus tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Progesterone Receptor (MA1-410) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Dog, Rabbit, Pig, Rat, Non-human primate, Bovine, Sheep, Human, Mouse, Guinea pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/mL|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-410 detects the progesterone receptor (PR) from human, non-human primate, bovine, rat, rabbit, and mouse tissues. This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor.
MA1-410 has been successfully used in Western blot, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from T47D whole cell lysate.
MA1-410 performs better by western blot using BSA as the blocking agent and in antibody diluent.
The MA1-410 immunogen is a synthetic peptide corresponding to the binding domain residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.
The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.
IP-MS enrichment of PGR (LFQ intensity): PGR was enriched 32-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and PGR antibody (Part No. MA1-410). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Progesterone receptor expression declines in the guinea pig uterus during functional progesterone withdrawal and in response to prostaglandins.
MA1-410 was used in western blot to assess progesterone receptor expression during late pregnancy and labor in vivo
|Welsh TN,Hirst JJ,Palliser H,Zakar T||PloS one (9:null)||2014|
Immunolocalization of progesterone receptors in the canine oviduct around ovulation.
MA1-410 was used in western blot to study the immunohistochemical expression of progesterone receptors in canine oviduct at various time-points around ovulation
|Tahir MZ,Reynaud K,Mawa G,Thoumire S,Chastant-Maillard S,Saint-Dizier M||Reproduction in domestic animals = Zuchthygiene (47 Suppl 6:35)||2012|
Expression of nuclear progesterone receptor and progesterone receptor membrane components 1 and 2 in the oviduct of cyclic and pregnant cows during the post-ovulation period.
MA1-410 was used in western blot to study the expression of nuclear progesterone receptor and of progesterone recptor membrane components 1 and 2 in bovine oviduct
|Saint-Dizier M,Sandra O,Ployart S,Chebrout M,Constant F||Reproductive biology and endocrinology : RB and E (10:null)||2012|
Progesterone receptor isoform expression in response to in utero growth restriction in the fetal guinea pig brain.
MA1-410 was used in western blot to study the effects of interuterine growth restriction of the expression of progesterone receptors A and B in fetal guinea pig brain
|Palliser HK,Yates DM,Hirst JJ||Neuroendocrinology (96:60)||2012|
Studies on the development of resistance to the pure antiestrogen Faslodex in three human breast cancer cell lines.
MA1-410 was used in western blot to investigate the mechanism for antiestrogen resistance in human breast cancer cell lines
|Sommer A,Hoffmann J,Lichtner RB,Schneider MR,Parczyk K||The Journal of steroid biochemistry and molecular biology (85:33)||2003|
Cloning and cellular localization of the canine progesterone receptor: co-localization with growth hormone in the mammary gland.
MA1-410 was used in western blot to investigate the localization of canine progesterone receptor
|Lantinga-van Leeuwen IS,van Garderen E,Rutteman GR,Mol JA||The Journal of steroid biochemistry and molecular biology (75:219)||2000|
Long-term hydroxytamoxifen treatment of an MCF-7-derived breast cancer cell line irreversibly inhibits the expression of estrogenic genes through chromatin remodeling.
MA1-410 was used in western blot to investigate the effect of tamoxifen long-term treatment on progesterone receptor expression.
|Badia E,Duchesne MJ,Semlali A,Fuentes M,Giamarchi C,Richard-Foy H,Nicolas JC,Pons M||Cancer research (60:4130)||2000|
No direct nuclear effect of progesterone in the inner ear: other possible pathways.
MA1-410 was used in immunohistochemistry to investigate the lack of progesterone receptor expression in rat and human inner ear
|Bonnard A,Sahlin L,Hultcrantz M,Simonoska R||Acta oto-laryngologica (133:1250)||2013|
Selective estrogen receptor-beta (SERM-beta) compounds modulate raphe nuclei tryptophan hydroxylase-1 (TPH-1) mRNA expression and cause antidepressant-like effects in the forced swim test.
MA1-410 was used in immunohistochemistry to study the modulation of raphe nuclei tryptophan hydroxylase-1 expression and mood by selective estrogen receptor-beta agonists in rodent models
|Clark JA,Alves S,Gundlah C,Rocha B,Birzin ET,Cai SJ,Flick R,Hayes E,Ho K,Warrier S,Pai L,Yudkovitz J,Fleischer R,Colwell L,Li S,Wilkinson H,Schaeffer J,Wilkening R,Mattingly E,Hammond M,Rohrer SP||Neuropharmacology (63:1051)||2012|
Intestinal tumorigenesis is not affected by progesterone signaling in rodent models.
MA1-410 was used in immunohistochemistry to study the lack of effect of progesterone receptor signaling in rodent models of intestinal tumorigenesis
|Heijmans J,Muncan V,Jacobs RJ,de Jonge-Muller ES,Graven L,Biemond I,Ederveen AG,Groothuis PG,Mosselman S,Hardwick JC,Hommes DW,van den Brink GR||PloS one (6:null)||2011|
Progesterone receptor immunoreactivity in the brains of ovariectomized aged rats.
MA1-410 was used in immunohistochemistry to investigate the distribution of progesterone receptor in ovariectomized rats of different ages
|Furuta M,Fukushima A,Chiba S,Sano A,Akema T,Kimura F,Funabashi T||Neuroreport (21:777)||2010|
Sex steroid receptor expression in the oviduct and uterus of sheep with estrus synchronized with progestagen or prostaglandin analogues.
MA1-410 was used in immunohistochemistry to investigate the impact of the treatment with progestagen and prostaglandin analogues on the expression of estrogen receptor-alpha, progesterone receptor and Ki-67 in the oviduct and uterus of sheep
|García-Palencia P,Sánchez MA,Nieto A,Vilar MP,González M,Veiga-Lopez A,González-Bulnes A,Flores JM||Animal reproduction science (97:25)||2007|
Overlapping and distinct expression of progesterone receptors A and B in mouse uterus and mammary gland during the estrous cycle.
MA1-410 was used in immunohistochemistry to detect the expression of progesterone receptors in mouse uterus and mammary gland
|Mote PA,Arnett-Mansfield RL,Gava N,deFazio A,Mulac-Jericevic B,Conneely OM,Clarke CL||Endocrinology (147:5503)||2006|
Tissue- and hormone-dependent progesterone receptor distribution in the rat uterus.
MA1-410 was used in immunohistochemistry to study the localization of PR in the rat uterus under various hormonal conditions
|Sahlin L,Masironi B,Akerberg S,Eriksson H||Reproductive biology and endocrinology : RB and E (4:null)||2006|
Long-term effects of fetal exposure to low doses of the xenoestrogen bisphenol-A in the female mouse genital tract.
MA1-410 was used in immunohistochemistry to study the effects of in utero exposure to low doses of bisphenol-A on the development of female reproductive tissues
|Markey CM,Wadia PR,Rubin BS,Sonnenschein C,Soto AM||Biology of reproduction (72:1344)||2005|
Coexistence of intracellular and membrane-bound progesterone receptors in human testis.
MA1-410 was used in immunohistochemistry to identify and characterize progesterone receptors in human testis.
|Shah C,Modi D,Sachdeva G,Gadkar S,Puri C||The Journal of clinical endocrinology and metabolism (90:474)||2005|
Progesterone receptor and the cell cycle modulate apoptosis in granulosa cells.
MA1-410 was used in immunohistochemistry to study the effect of progesterone receptor and the cell cycle on granulosa cell apoptosis.
|Quirk SM,Cowan RG,Harman RM||Endocrinology (145:5033)||2004|
Development and characterization of an endometrial tissue culture system.
MA1-410 was used in immunohistochemistry to develop a system for the culture of whole pieces of endometrium
|Stavreus-Evers A,Hovatta O,Eriksson H,Landgren BM||Reproductive biomedicine online (7:243)||2003|
Effects of an antiprogestin onapristone on the endometrium of bonnet monkeys: morphometric and ultrastructural studies.
MA1-410 was used in immunohistochemistry to study how the long-term low-dose onapristone treatment affects endometrial development at the subcellular level
|Gopalkrishnan K,Katkam RR,Sachdeva G,Kholkute SD,Padwal V,Puri CP||Biology of reproduction (68:1959)||2003|
Multifocal ductal cell hyperplasia in the submandibular salivary glands of Wistar rats chronically treated with a novel steroidal compound.
MA1-410 was used in immunohistochemistry to investigate the formation of multifocal hyperplastic ductal lesions in rat submandibular salivary gland after chronical treatment with a novel synthetic steroid
|de Rijk EP,Ravesloot WT,Hafmans TG,van Esch E||Toxicologic pathology (31:1)||2003|
Expression and localization of estrogen receptor alpha, estrogen receptor beta and progesterone receptor in the bovine oviduct in vivo and in vitro.
MA1-410 was used in immunohistochemistry to investigate the expression and localization of estrogen receptor alpha and beta and progesterone receptor in bovine oviducts
|Ulbrich SE,Kettler A,Einspanier R||The Journal of steroid biochemistry and molecular biology (84:279)||2003|
Expression of progesterone receptors A and B and insulin-like growth factor-I in human myometrium and fibroids after treatment with a gonadotropin-releasing hormone analogue.
MA1-410 was used in immunohistochemistry to study the effects of a gonadotrophin-releasing hormone on the expression of progesterone receptors A and B and IGF-1 in human myometrium and fibroids
|Wu X,Wang H,Englund K,Blanck A,Lindblom B,Sahlin L||Fertility and sterility (78:985)||2002|
Immunodetection of nmt55/p54nrb isoforms in human breast cancer.
MA1-410 was used in immunohistochemistry to investigate the expression level of nmt55/p54nrb isoforms in human breast cancer
|Pavao M,Huang YH,Hafer LJ,Moreland RB,Traish AM||BMC cancer (1:null)||2002|
Cellular turnover in the rat uterine cervix and its relationship to estrogen and progesterone receptor dynamics.
MA1-410 was used in immunohistochemistry to investigate the cell proliferation and apoptosis in relationship with sex steroid hormone receptors in the rat uterine cervix during the estrous cycle, pregnancy, and postpartum
|Ramos JG,Varayoud J,Bosquiazzo VL,Luque EH,Muñoz-de-Toro M||Biology of reproduction (67:735)||2002|
Formation of pinopodes in human endometrium is associated with the concentrations of progesterone and progesterone receptors.
MA1-410 was used in immunohistochemistry to study the relationship between the development of human endometrial pinopodes and the levels of progesterone, progesterone receptors and estrogen receptors
|Stavreus-Evers A,Nikas G,Sahlin L,Eriksson H,Landgren BM||Fertility and sterility (76:782)||2001|
The effect of progestins on vascular endothelial growth factor, oestrogen receptor and progesterone receptor immunoreactivity and endothelial cell density in human endometrium.
MA1-410 was used in immunohistochemistry to investigate the effect of progestin on the expression of VEGF, ER and PR in relation to endometrial bleeding
|Charnock-Jones DS,Macpherson AM,Archer DF,Leslie S,Makkink WK,Sharkey AM,Smith SK||Human reproduction (Oxford, England) (15 Suppl 3:85)||2000|
Multiple roles for heparin-binding epidermal growth factor-like growth factor are suggested by its cell-specific expression during the human endometrial cycle and early placentation.
MA1-410 was used in immunohistochemistry to study the roles of heparin-binding epidermal growth factor-like growth factor during the human endometrial cycle and early placentation
|Leach RE,Khalifa R,Ramirez ND,Das SK,Wang J,Dey SK,Romero R,Armant DR||The Journal of clinical endocrinology and metabolism (84:3355)||1999|
Segregation of steroid receptor coactivator-1 from steroid receptors in mammary epithelium.
MA1-410 was used in immunohistochemistry to study the interactions between steroid receptor coactivator-1 and steroid receptors in mammary epithelium.
|Shim WS,DiRenzo J,DeCaprio JA,Santen RJ,Brown M,Jeng MH||Proceedings of the National Academy of Sciences of the United States of America (96:208)||1999|
Effects of interferon-tau and progesterone on oestrogen-stimulated expression of receptors for oestrogen, progesterone and oxytocin in the endometrium of ovariectomized ewes.
MA1-410 was used in immunohistochemistry to investigate the impacts of interferon tau and progesterone on endometrial expression of estrogen receptor, progesterone receptor and OTR in ovariectomized ewes
|Spencer TE,Mirando MA,Mayes JS,Watson GH,Ott TL,Bazer FW||Reproduction, fertility, and development (8:843)||1997|
Effect of estrogen on ontogenic expression of progesterone and estrogen receptors in rat uterus.
MA1-410 was used in immunohistochemistry to investigate the influence of estrogen on progesterone recepor and estrogen receptor expression in the rat uterus
|Ohta Y,Fukazawa Y,Sato T,Suzuki A,Nishimura N,Iguchi T||Zoological science (13:143)||1996|
Estrogen and progesterone receptors, cell proliferation, and c-fos expression in the ovine uterus during early pregnancy.
MA1-410 was used in immunohistochemistry to investigate the importance of steroid receptors in cell growth in ovine uterus
|Zheng J,Johnson ML,Redmer DA,Reynolds LP||Endocrinology (137:340)||1996|
Temporal and spatial alterations in uterine estrogen receptor and progesterone receptor gene expression during the estrous cycle and early pregnancy in the ewe.
MA1-410 was used in immunohistochemistry to profile the gene expression of uterine estrogen receptor and progesterone receptor in the ewe
|Spencer TE,Bazer FW||Biology of reproduction (53:1527)||1995|
Ovine interferon-tau regulates expression of endometrial receptors for estrogen and oxytocin but not progesterone.
MA1-410 was used in immunohistochemistry to study the IFN-tau's ability to stablize PR expression in the enodmetrium
|Spencer TE,Becker WC,George P,Mirando MA,Ogle TF,Bazer FW||Biology of reproduction (53:732)||1995|
Subfertility linked to combined luteal insufficiency and uterine progesterone resistance.
MA1-410 was used in immunocytochemistry to investigate the cause of subfertility in the vrown Norway rat
|Konno T,Graham AR,Rempel LA,Ho-Chen JK,Alam SM,Bu P,Rumi MA,Soares MJ||Endocrinology (151:4537)||2010|
Sexual behavior in lactating rats: role of estrogen-induced progesterone receptors.
MA1-410 was used in immunocytochemistry to investigate the estrogen-induced progesterone receptors in lactating rats
|Barbosa-Vargas E,Pfaus JG,Woodside B||Hormones and behavior (56:246)||2009|
Characterisation of steroid receptor expression in the human prostate carcinoma cell line 22RV1 and quantification of androgen effects on mRNA regulation of prostate-specific genes.
MA1-410 was used in immunocytochemistry to investigate the impact of androgens on steroid receptor expression in 22RV1 cells
|Hartel A,Didier A,Ulbrich SE,Wierer M,Meyer HH||The Journal of steroid biochemistry and molecular biology (92:187)||2004|
Immunocytochemical detection of progesterone receptor in the female rabbit forebrain: distribution and regulation by oestradiol and progesterone.
MA1-410 was used in immunocytochemistry to investigate the localization of progesterone receptor in ovariectomized female rabbit forebrain by immunocytochemistry
|Caba M,Rovirosa MJ,Beyer C,González-Mariscal G||Journal of neuroendocrinology (15:855)||2003|
Characterization of reproductive steroid receptors and response to estrogen in a rat serotonergic cell line.
MA1-410 was used in immunocytochemistry to study the expression and function of reproductive steroid receptors in a rat serotonergic cell line
|Bethea CL,Lu NZ,Reddy A,Shlaes T,Streicher JM,Whittemore SR||Journal of neuroscience methods (127:31)||2003|
Bisphenol A increases progesterone receptor immunoreactivity in the hypothalamus in a dose-dependent manner and affects sexual behaviour in adult ovariectomized rats.
MA1-410 was used in immunocytochemistry to investigate the influence of estrogen receptor on progesterone receptor expression in rat hypothalamus
|Funabashi T,Sano A,Mitsushima D,Kimura F||Journal of neuroendocrinology (15:134)||2003|
Progesterone receptor as an indicator of sperm function.
MA1-410 was used in immunocytochemistry to investigate role of PR in the events leading to the acrosome reaction in sperm
|Gadkar S,Shah CA,Sachdeva G,Samant U,Puri CP||Biology of reproduction (67:1327)||2002|
Ovarian steroid action on tryptophan hydroxylase protein and serotonin compared to localization of ovarian steroid receptors in midbrain of guinea pigs.
MA1-410 was used in immunocytochemistry to investigate the effect of ovarian steroid on serotonin synthesis in the midbrain of guinea pigs
|Lu NZ,Shlaes TA,Gundlah C,Dziennis SE,Lyle RE,Bethea CL||Endocrine (11:257)||1999|
Expression of intracellular progesterone receptors in rat brain during different reproductive states, and involvement in maternal behavior.
MA1-410 was used in immunocytochemistry to investigate the expression of progesterone receptors in rat brain and its role in maternal behavior
|Numan M,Roach JK,del Cerro MC,Guillamón A,Segovia S,Sheehan TP,Numan MJ||Brain research (830:358)||1999|
Gene expression profiles and structural/functional features of the peri-implantation endometrium in natural and gonadotropin-stimulated cycles.
MA1-410 was used in immunoprecipitation to study the effect of controlled ovarian hyperstimulation on gene expression of the peri-implantation endometrium .
|Mirkin S,Nikas G,Hsiu JG,Díaz J,Oehninger S||The Journal of clinical endocrinology and metabolism (89:5742)||2004|