|ChIP assay (ChIP)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/mL|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200-1:1000|
|Western Blot (WB)||See 7 publications below|
|Immunohistochemistry (IHC)||See 27 publications below|
|Immunocytochemistry (ICC)||See 9 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Dog , Rabbit , Pig , Rat , Non-human primate , Bovine , Sheep , Human , Mouse Guinea pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-410 detects the progesterone receptor (PR) from human, non-human primate, bovine, rat, rabbit, and mouse tissues. This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor.
MA1-410 has been successfully used in Western blot, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from T47D whole cell lysate.
MA1-410 performs better by western blot using BSA as the blocking agent and in antibody diluent.
The MA1-410 immunogen is a synthetic peptide corresponding to the binding domain residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.
The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: NR3C3; Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor
Gene Aliases: 9930019P03Rik; BB114106; ENSMUSG00000074510; NR3C3; PGR; PR; PR-A; PR-B
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