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Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Progesterone Receptor mouse monoclonal antibody (Product # MA5-12581), at a dilution of 1:100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 Superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Formalin fixed recombinant full length A-form of human progesterone receptor|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:20|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA5-12581 targets Progesterone Receptor in IHC (P) applications and shows reactivity with Human samples.
The MA5-12581 immunogen is formalin fixed recombinant full length A-form of human progesterone receptor.
MA5-12581 detects A and B forms of progesterone receptor from human and mouse in western blot, immunofluorescence and immunohistochemistry.
Human PgR exists in two forms: 116kDa (B-form) and 81kDa (A-form). It acts as ligand activated transcription factor to regulate expression of the target genes. Null mutation in PgR gene leads to pleiotrophic reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Analysis of peptidyl-propyl-cis/trans isomerase 1 (PIN1) gene -842(G > C) and -667(T > C) polymorphic variants in relation to breast cancer risk and clinico-pathological parameters.
MA5-12581 was used in immunohistochemistry to analyze the polymorphism of peptidyl-propyl-cis/trans isomerase 1 in breast cancer
|Naidu R,Har YC,Taib NA||Scandinavian journal of clinical and laboratory investigation (71:500)||2011|
Expression of KAI1/CD82 in distant metastases from estrogen receptor-negative breast cancer.
MA5-12581 was used in immunohistochemistry to examine the prognostic significance of KAI1/CD82 in estrogen receptor-negative breast cancer
|Christgen M,Christgen H,Heil C,Krech T,Länger F,Kreipe H,Lehmann U||Cancer science (100:1767)||2009|
nuclear receptor subfamily 3 group C member 3
NR3C3; PGR; PR