Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with or without Progesterone Receptor mouse monoclonal antibody (Product # MA1-412), at a dilution of 1:100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 Superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Chicken, Human, Reptile|
|Published species reactivity||Rabbit, Human, Mouse, Chicken|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Progesterone receptor purified from chick oviduct cytosol.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Gel Shift (GS)||Assay Dependent|
|Immunofluorescence (IF)||10 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-412 detects the A and B forms of progesterone receptor from chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.
MA1-412 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 78 kDa protein representing the A form and a 110 kDa protein representing the B form of PR. The proportion of receptor bound by this product is significantly greater than catalog number MA1-411 due to the binding of both the A and B form of PR. Protein A can effectively be used with this product in immunoprecipitation experiments.
MA1-412 detects the A and B forms of progesterone receptor from human, chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.
The MA1-412 immunogen is progesterone receptor purified from chick oviduct cytosol.
Reconstitute with PBS.
The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Uterine epithelial morphology and progesterone receptors in a mifepristone-treated viviparous lizard Pseudemoia entrecasteauxii (Squamata: Scincidae) during gestation.
MA1-412 was used in western blot to study the uterine epithelial morphology and progesterone receptors in a viviparous lizard during gestation
|Biazik JM,Parker SL,Murphy CR,Thompson MB||Journal of experimental zoology. Part B, Molecular and developmental evolution (318:148)||2012|
Progesterone receptor deficient in chromatin binding has an altered cellular state.
MA1-412 was used in immunoprecipitation to analyze the effect of progesterone receptors deficient in chromatin binding.
|Botos J,Xian W,Smith DF,Smith CL||The Journal of biological chemistry (279:15231)||2004|
The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties.
MA1-412 was used in immunoprecipitation to investigate TRAP1 functional properties as a mitochondrial protein
|Felts SJ,Owen BA,Nguyen P,Trepel J,Donner DB,Toft DO||The Journal of biological chemistry (275:3305)||2000|
Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.
MA1-412 was used in immunoprecipitation to investigate the important role of Hip's C-terminal region in progesterone receptor assembly
|Prapapanich V,Chen S,Smith DF||Molecular and cellular biology (18:944)||1998|
Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids.
MA1-412 was used in immunoprecipitation to investigate the mechanism of hepadnavirus assembly and viral DNA synthesis
|Hu J,Toft DO,Seeger C||The EMBO journal (16:59)||1997|
Molecular cloning of human p48, a transient component of progesterone receptor complexes and an Hsp70-binding protein.
MA1-412 was used in immunoprecipitation to characterize the interactions of human p48 in progesterone recepor complexes and with different kinds of heat shock proteins
|Prapapanich V,Chen S,Nair SC,Rimerman RA,Smith DF||Molecular endocrinology (Baltimore, Md.) (10:420)||1996|
A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23.
MA1-412 was used in immunoprecipitation to investigate the composition of a new kind of chaperone complex for steroid receptors
|Johnson JL,Toft DO||The Journal of biological chemistry (269:24989)||1994|
Immunocytochemical study of progesterone receptors in hyperplastic and neoplastic endometrial tissues.
MA1-412 was used in immunoprecipitation to investigate the expression and distribution of progesterone receptors in hyperplastic and neoplastic human endometrium
|Bergeron C,Ferenczy A,Toft DO,Shyamala G||Cancer research (48:6132)||1988|