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Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with or without Progesterone Receptor mouse monoclonal antibody (Product # MA5-12658), at a concentration of 10ug/ml for 1 hour at 37C, and then incubated with a Alexa Fluor 488 Superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||PgR from a human endometrial carcinoma (EnCa 101) grown in athymic mice|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||10 µg|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12658 targets Progesterone Receptor in IF, IP, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-12658 immunogen is pgR from a human endometrial carcinoma (EnCa 101) grown in athymic mice.
Human PgR exists in two forms: 116kDa (B-form) and 81kDa (A-form). It acts as ligand activated transcription factor to regulate expression of the target genes. Null mutation in PgR gene leads to pleiotrophic reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amphiregulin mediates progesterone-induced mammary ductal development during puberty.
MA5-12658 was used in immunohistochemistry to study the modulation of pubertal progesterone-induced development of the mammary ducts by amphiregulin
|Aupperlee MD,Leipprandt JR,Bennett JM,Schwartz RC,Haslam SZ||Breast cancer research : BCR (15:null)||2014|
Hypermethylation of the progesterone receptor A in constitutive antiprogestin-resistant mouse mammary carcinomas.
MA5-12658 was used in immunohistochemistry and western blot to study the role of hypermethylation of the progesterone receptor A in constitutive antiprogestin-resistant mouse mammary carcinomas
|Wargon V,Fernandez SV,Goin M,Giulianelli S,Russo J,Lanari C||Breast cancer research and treatment (126:319)||2011|
Age-related changes in the epithelial and stromal compartments of the mammary gland in normocalcemic mice lacking the vitamin D3 receptor.
MA5-12658 was used in immunohistochemistry to study the effect of vitamin D3 receptor knock out on the mammary gland in normocalcemic mice
|Welsh J,Zinser LN,Mianecki-Morton L,Martin J,Waltz SE,James H,Zinser GM||PloS one (6:null)||2011|
Tip30 deletion in MMTV-Neu mice leads to enhanced EGFR signaling and development of estrogen receptor-positive and progesterone receptor-negative mammary tumors.
MA5-12658 was used in immunohistochemistry to study the mechanism by which Tip30 loss leads to development of mammary tumors
|Zhang C,Mori M,Gao S,Li A,Hoshino I,Aupperlee MD,Haslam SZ,Xiao H||Cancer research (70:10224)||2010|
Inhibition of mammary tumor growth by estrogens: is there a specific role for estrogen receptors alpha and beta?
MA5-12658 was used in immunohistochemistry to determine if estrogen influences tumor growth via stimulation of estrogen receptors
|Soldati R,Wargon V,Cerliani JP,Giulianelli S,Vanzulli SI,Gorostiaga MA,Bolado J,do Campo P,Molinolo A,Vollmer G,Lanari C||Breast cancer research and treatment (123:709)||2010|
Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis.
MA5-12658 was used in immunohistochemistry to study the role of cancer-associated fibroblasts in mammary tumorigenesis and development of hormone independence
|Giulianelli S,Cerliani JP,Lamb CA,Fabris VT,Bottino MC,Gorostiaga MA,Novaro V,Góngora A,Baldi A,Molinolo A,Lanari C||International journal of cancer (123:2518)||2008|
Prognosis of uterine corpus cancer after tamoxifen treatment for breast cancer.
MA5-12658 was used in immunohistochemistry to study the effect of tamoxifen treatment of breast cancer on the prognosis of uterine corpus cancer
|Hoogendoorn WE,Hollema H,van Boven HH,Bergman E,de Leeuw-Mantel G,Platteel I,Fles R,Nederlof PM,Mourits MJ,van Leeuwen FE||Breast cancer research and treatment (112:99)||2008|
Progesterone receptor isoforms and proliferation in the rat mammary gland during development.
MA5-12658 was used in immunohistochemistry to study the role of progesterone receptor isoforms in rat mammary gland proliferation during development
|Kariagina A,Aupperlee MD,Haslam SZ||Endocrinology (148:2723)||2007|
Expression profiles of sex steroid receptors in desmoid tumors.
MA5-12658 was used in immunohistochemistry to study the expression of estrogen, progesterone and androgen receptors in desmoid tumors
|Ishizuka M,Hatori M,Dohi O,Suzuki T,Miki Y,Tazawa C,Sasano H,Kokubun S||The Tohoku journal of experimental medicine (210:189)||2006|
Disappearance of steroid hormone dependency during malignant transformation of ovarian clear cell cancer.
MA5-12658 was used in immunohistochemistry to study the loss of steroid hormone dependency during malignant transformation of ovarian clear cell cancer
|Akahane T,Sekizawa A,Okuda T,Kushima M,Saito H,Okai T||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (24:369)||2005|
Phosphorylation of estrogen receptor alpha serine 167 is predictive of response to endocrine therapy and increases postrelapse survival in metastatic breast cancer.
MA5-12658 was used in immunohistochemistry to study the value of estrogen receptor alpha serine 167 phosphorylation in predicting the response to endocrine therapy and postrelapse survival in metastatic breast cancer
|Yamashita H,Nishio M,Kobayashi S,Ando Y,Sugiura H,Zhang Z,Hamaguchi M,Mita K,Fujii Y,Iwase H||Breast cancer research : BCR (7:R753)||2005|
Progesterone receptor isoform (A/B) ratio of human fetal membranes increases during term parturition.
MA5-12658 was used in immunohistochemistry to study changes in the progesterone receptor isoform (A/B) ratio of human fetal membranes during term parturition
|Oh SY,Kim CJ,Park I,Romero R,Sohn YK,Moon KC,Yoon BH||American journal of obstetrics and gynecology (193:1156)||2005|
Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality.
MA5-12658 was used in immunohistochemistry to study the relationship between progesterone receptor expression in human cumulus cells and developmental competence of human oocytes during IVF treatment
|Hasegawa J,Yanaihara A,Iwasaki S,Otsuka Y,Negishi M,Akahane T,Okai T||Human reproduction (Oxford, England) (20:2194)||2005|
Progestins induce transcriptional activation of signal transducer and activator of transcription 3 (Stat3) via a Jak- and Src-dependent mechanism in breast cancer cells.
MA5-12658 was used in immunohistochemistry and western blot to study the role of Jaj and Src in the transcriptional activation of Stat3 by progestins in breast cancer cells
|Proietti C,Salatino M,Rosemblit C,Carnevale R,Pecci A,Kornblihtt AR,Molinolo AA,Frahm I,Charreau EH,Schillaci R,Elizalde PV||Molecular and cellular biology (25:4826)||2005|
Cytologic scoring of endometrioid adenocarcinoma of the endometrium.
MA5-12658 was used in immunohistochemistry to study the usefulness of a cytologic scoring system for endometrioid adenocarcinoma
|Nishimura Y,Watanabe J,Jobo T,Hattori M,Arai T,Kuramoto H||Cancer (105:8)||2005|
Nuclear receptor DAX-1 in human common epithelial ovarian carcinoma: an independent prognostic factor of clinical outcome.
MA5-12658 was used in immunohistochemistry to study the prognostic value of nuclear receptor DAX-1 in human common epithelial ovarian carcinoma
|Abd-Elaziz M,Akahira J,Moriya T,Suzuki T,Yaegashi N,Sasano H||Cancer science (94:980)||2003|
Expansion of CD11b(+)Ly6G (+)Ly6C (int) cells driven by medroxyprogesterone acetate in mice bearing breast tumors restrains NK cell effector functions.
MA5-12658 was used in western blot to study the effects of breast tumor exposure to medroxyprogesterone on myeloid-derived suppressor cells and NK cells
|Spallanzani RG,Dalotto-Moreno T,Raffo Iraolagoitia XL,Ziblat A,Domaica CI,Avila DE,Rossi LE,Fuertes MB,Battistone MA,Rabinovich GA,Salatino M,Zwirner NW||Cancer immunology, immunotherapy : CII (62:1781)||2013|
Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer.
MA5-12658 was used in western blot to study the novel role of Stat3 as a progesterone receptor coactivator in breast cancer.
|Proietti CJ,Béguelin W,Flaqué MC,Cayrol F,Rivas MA,Tkach M,Charreau EH,Schillaci R,Elizalde PV||Steroids (76:381)||2011|
Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth.
MA5-12658 was used in western blot to investigate the role of Stat3 in the tumorigenic effect of heregulin/ErbB-2 in breast cells
|Proietti CJ,Rosemblit C,Beguelin W,Rivas MA,Díaz Flaqué MC,Charreau EH,Schillaci R,Elizalde PV||Molecular and cellular biology (29:1249)||2009|
Inhibition of in vivo breast cancer growth by antisense oligodeoxynucleotides to type I insulin-like growth factor receptor mRNA involves inactivation of ErbBs, PI-3K/Akt and p42/p44 MAPK signaling pathways but not modulation of progesterone receptor activity.
MA5-12658 was used in western blot to study the mechanism by which antisense oligodeoxynucleotides to type I IGF receptor mRNA inhibit in vivo breast cancer growth
|Salatino M,Schillaci R,Proietti CJ,Carnevale R,Frahm I,Molinolo AA,Iribarren A,Charreau EH,Elizalde PV||Oncogene (23:5161)||2004|
Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice.
MA5-12658 was used in western blot to study the role of aromatase in the biochemical pathways leading to mammary tumorigenesis in transgenic mice
|Kirma N,Gill K,Mandava U,Tekmal RR||Cancer research (61:1910)||2001|
Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3.
MA5-12658 was used in ChIP assay to study how progesterone receptor-induced nuclear translocation of ErbB-2 promotes breast cancer growth.
|Béguelin W,Díaz Flaqué MC,Proietti CJ,Cayrol F,Rivas MA,Tkach M,Rosemblit C,Tocci JM,Charreau EH,Schillaci R,Elizalde PV||Molecular and cellular biology (30:5456)||2010|
Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells.
MA5-12658 was used in immunocytochemistry to investigate the influence of catechol-o-methyltransferase and 2-methoxyestradiol on cytoskeleton structure and steroid receptor signaling
|Salama SA,Kamel MW,Botting S,Salih SM,Borahay MA,Hamed AA,Kilic GS,Saeed M,Williams MY,Diaz-Arrastia CR||PloS one (4:null)||2009|
Gene expression programs of human smooth muscle cells: tissue-specific differentiation and prognostic significance in breast cancers.
MA5-12658 was used in immunocytochemistry to study the gene expression programs of human smooth muscle cells
|Chi JT,Rodriguez EH,Wang Z,Nuyten DS,Mukherjee S,van de Rijn M,van de Vijver MJ,Hastie T,Brown PO||PLoS genetics (3:1770)||2007|
Medroxyprogesterone acetate enhances in vivo and in vitro antibody production.
MA5-12658 was used in flow cytometry to study the mechanism by which medroxyprogesterone acetate enhances in vivo and in vitro antibody production
|Vermeulen M,Pazos P,Lanari C,Molinolo A,Gamberale R,Geffner JR,Giordano M||Immunology (104:80)||2001|
Identification of progesterone receptor in human subcutaneous adipose tissue.
MA5-12658 was used in ELISA to study the presence of the progesterone receptor in human subcutaneous adipose tissue
|O'Brien SN,Welter BH,Mantzke KA,Price TM||The Journal of clinical endocrinology and metabolism (83:509)||1998|
nuclear receptor subfamily 3 group C member 3; PR; progesterone receptor
9930019P03Rik; BB114106; ENSMUSG00000074510; NR3C3; PGR; PR; PR-A; PR-B