Immunohistochemistry analysis of progranulin was performed on human kidney tissue and is showing positive staining (left panel). To expose target proteins, antigen retrieval was performed by microwaving tissues for 15 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with progranulin monoclonal antibody (Product # MA1-187) at a dilution of 1:20, over night at 4C in a humidified chamber without (right panel) or with progranulin antibody (left panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using a DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human Progranulin|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
By Western blot, MA1-187 detects Human Progranulin at ~85 kDa.
Progranulin is the precursor protein of Granulin also called as Proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature Granulin. Both Granulin and smaller cleavage product of Granulin regulates cell growth. Progranulin stimulates proliferation of many epithelial cells, promotes their anchorage-independent growth and, when overexpressed, confers epithelial tumorigenicity. Progranulin/epithelin gene is multifunctional, with important constitutive roles in epithelial homeostasis, reproductive, immunological, and neuronal function. Progranulin constitutively expressed in a number of epithelia, particularly in the skin, GI tract, and reproductive system. Progranulin is expressed in immune cells in vivo and in specific neurons in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.