Immunofluorescent analysis of Prolactin Receptor in H-4-II-E Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody (Product # MA1-611) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified rat liver PRL receptor.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-611 detects prolactin (PRL) receptor in rat and human tissues. This antibody does not cross-react with PRL receptors from other species nor with growth hormone (GH) receptor.
MA1-611 has been successfully used in Western blot, IF, immunohistochemistry (4% PFA fixed tissue), and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells with MA1-611 yields a staining pattern consistent with cytoplasmic vesicular staining. MA1-611 has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.
The MA1-611 immunogen is purified rat liver PRL receptor.
Reconstitute with 100 ul PBS.
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa.
The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Prolactin secretory rhythm of mated rats induced by a single injection of oxytocin.
MA1-611 was used in immunohistochemistry to study the effect of oxytocin on prolactin secretory rhythm in rats.
|Egli M,Bertram R,Toporikova N,Sellix MT,Blanco W,Freeman ME||American journal of physiology. Endocrinology and metabolism (290:E566)||2006|
Hypothesis paper Brain talks with fat--evidence for a hypothalamic-pituitary-adipose axis?
MA1-611 was used in western blot to investigate the involvement of adipose tissue in hypothalamic-pituitary-adipose axis
|Schäffler A,Binart N,Schölmerich J,Büchler C||Neuropeptides (39:363)||2005|
|Rat||10^7 cpm:50 ml||
Mutational analysis of the ligand-binding domain of the prolactin receptor.
MA1-611 was used in western blot to perform mutational analysis of prolactin receptor in order to investigate the contributions of different domains to hormone binding
|Rozakis-Adcock M,Kelly PA||The Journal of biological chemistry (266:16472)||1991|