Immunofluorescent analysis of Prolactin Receptor using Prolactin Receptor Monoclonal Antibody (U5) (Product# MA1-610 ) shows staining in A2058 Cells. Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor (Product# MA1-610 ) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Horse, Human, Pig, Rabbit, Rat|
|Published species reactivity||Rabbit, Rat, Non-human primate, Bovine, Hamster, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified rat liver PRL receptor.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Western Blot (WB)||See 12 publications below|
|Immunohistochemistry (IHC)||See 8 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues.
MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor.
The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor.
Reconstitute with 100 ul PBS.
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa.
The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways.
MA1-610 was used in immunocytochemistry to study the age-related effects of prolactin and growth hormone on bovine oocytes
|Lebedeva IY,Singina GN,Lopukhov AV,Shedova EN,Zinovieva NA||Frontiers in genetics (6:null)||2015|
Prolactin affects bovine oocytes through direct and cumulus-mediated pathways.
MA1-610 was used in immunocytochemistry to characterize pathways of PRL action on bovine oocytes
|Lebedeva IY,Singina GN,Volkova NA,Vejlsted M,Zinovieva NA,Schmidt M||Theriogenology (82:1154)||2014|
Prolactin-regulated tyrosine hydroxylase activity and messenger ribonucleic acid expression in mediobasal hypothalamic cultures: the differential role of specific protein kinases.
MA1-610 was used in immunocytochemistry to investigate the different roles of protein kinases in prolactin-regulated tyrosine hydroxylase activity.
|Ma FY,Grattan DR,Goffin V,Bunn SJ||Endocrinology (146:93)||2005|
Response of Diamond-Blackfan anemia to metoclopramide: evidence for a role for prolactin in erythropoiesis.
MA1-610 was used in immunocytochemistry to study the role of prolactin during the erythropoiesis
|Abkowitz JL,Schaison G,Boulad F,Brown DL,Buchanan GR,Johnson CA,Murray JC,Sabo KM||Blood (100:2687)||2002|
Constitutive tyrosine phosphorylation of ErbB-2 via Jak2 by autocrine secretion of prolactin in human breast cancer.
MA1-610 was used in immunocytochemistry to investigate the mechanism for constitutive tyrosine-phosphorylation of ErbB2 in human breast cancer
|Yamauchi T,Yamauchi N,Ueki K,Sugiyama T,Waki H,Miki H,Tobe K,Matsuda S,Tsushima T,Yamamoto T,Fujita T,Taketani Y,Fukayama M,Kimura S,Yazaki Y,Nagai R,Kadowaki T||The Journal of biological chemistry (275:33937)||2000|
Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation.
MA1-610 was used in immunocytochemistry to investigate the mechanism for the internalization of prolactin and its receptor
|Perrot-Applanat M,Gualillo O,Buteau H,Edery M,Kelly PA||Journal of cell science (110 ( Pt 9):1123)||1997|
Mechanisms of transient signaling via short and long prolactin receptor isoforms in female and male sensory neurons.
MA1-610 was used in immunohistochemistry and western blot to study transient prolactin signaling mediated by the short and the long forms of the prolactin receptor in sensory neurons of male and female rats
|Belugin S,Diogenes AR,Patil MJ,Ginsburg E,Henry MA,Akopian AN||The Journal of biological chemistry (288:34943)||2013|
Prolactin does not affect human platelet aggregation or secretion.
MA1-610 was used in western blot to study the lack of effect of prolactin on human platelet function
|Reuwer AQ,Nieuwland R,Fernandez I,Goffin V,van Tiel CM,Schaap MC,Berckmans RJ,Kastelein JJ,Twickler MT||Thrombosis and haemostasis (101:1119)||2009|
Prolactin activates mitogen-activated protein kinase signaling and corticotropin releasing hormone transcription in rat hypothalamic neurons.
MA1-610 was used in western blot to study the effect of prolactin on MAP kinase signaling and CRH transcription in rat hypothalamic neurons.
|Blume A,Torner L,Liu Y,Subburaju S,Aguilera G,Neumann ID||Endocrinology (150:1841)||2009|
Prolactin prevents chronic stress-induced decrease of adult hippocampal neurogenesis and promotes neuronal fate.
MA1-610 was used in western blot to study the role of prolactin in the neurogenesis in hippocampal dentate gyrus.
|Torner L,Karg S,Blume A,Kandasamy M,Kuhn HG,Winkler J,Aigner L,Neumann ID||The Journal of neuroscience : the official journal of the Society for Neuroscience (29:1826)||2009|
|Non-human primate||Not Cited||
Overexpression of the mTOR alpha4 phosphoprotein activates protein phosphatase 2A and increases Stat1alpha binding to PIAS1.
MA1-610 was used in western blot to study the activation of protein phosphatase 2A by the mTOR alpha4 phosphoprotein
|Nien WL,Dauphinee SM,Moffat LD,Too CK||Molecular and cellular endocrinology (263:10)||2007|
A novel estradiol/estrogen receptor alpha-dependent transcriptional mechanism controls expression of the human prolactin receptor.
MA1-610 was used in western blot to study the mechanism for estradiol-induced human prolactin receptor expression.
|Dong J,Tsai-Morris CH,Dufau ML||The Journal of biological chemistry (281:18825)||2006|
ERK3 associates with MAP2 and is involved in glucose-induced insulin secretion.
MA1-610 was used in western blot to study the role of ERK3 and MAP2 in glucose-induced insulin secretion
|Anhê GF,Torrão AS,Nogueira TC,Caperuto LC,Amaral ME,Medina MC,Azevedo-Martins AK,Carpinelli AR,Carvalho CR,Curi R,Boschero AC,Bordin S||Molecular and cellular endocrinology (251:33)||2006|
Src family kinases are required for prolactin induction of cell proliferation.
MA1-610 was used in western blot to study the role of Src family of tyrosine kinases (SFK) on PRL-induced cell proliferation
|Fresno Vara JA,Cáceres MA,Silva A,Martín-Pérez J||Molecular biology of the cell (12:2171)||2001|
Suppression of nerve growth factor Trk receptors and prolactin receptors by endocannabinoids leads to inhibition of human breast and prostate cancer cell proliferation.
MA1-610 was used in western blot to investigate whether and through what mechanism anandamide and 2-AG inhibit the nerve growth factor-induced proliferation of HBCCs and the PRL-induced proliferation of human prostate cancer cells.
|Melck D,De Petrocellis L,Orlando P,Bisogno T,Laezza C,Bifulco M,Di Marzo V||Endocrinology (141:118)||2000|
Null mutation of the prolactin receptor gene produces multiple reproductive defects in the mouse.
MA1-610 was used in western blot to investigate the consequences of null mutation of the prolactin receptor with respect to reproduction in a mouse model
|Ormandy CJ,Camus A,Barra J,Damotte D,Lucas B,Buteau H,Edery M,Brousse N,Babinet C,Binart N,Kelly PA||Genes and development (11:167)||1997|
|Rat||10^7 cpm:50 ml||
Mutational analysis of the ligand-binding domain of the prolactin receptor.
MA1-610 was used in western blot to perform mutational analysis of prolactin receptor in order to investigate the contributions of different domains to hormone binding
|Rozakis-Adcock M,Kelly PA||The Journal of biological chemistry (266:16472)||1991|
Purification and protein sequence analysis of rat liver prolactin receptor.
MA1-610 was used in western blot to characterize prolactin receptors in rat liver
|Okamura H,Raguet S,Bell A,Gagnon J,Kelly PA||The Journal of biological chemistry (264:5904)||1989|
Body reserves and ovarian performance in primiparous lactating rabbit does submitted to early weaning as a strategy to decrease energy deficit.
MA1-610 was used in immunohistochemistry to study the effect of early weaning on metabolism and ovarian function in rabbits.
|Sakr OG,García-García RM,Arias-Alvarez M,Millán P,Lorenzo PL,Rebollar PG||Animal reproduction science (121:294)||2010|
Prolactin is involved in glial responses following a focal injury to the juvenile rat brain.
MA1-610 was used in immunohistochemistry to study the role of prolactin in glial responses following a focal injury to the juvenile rat brain
|Möderscheim TA,Gorba T,Pathipati P,Kokay IC,Grattan DR,Williams CE,Scheepens A||Neuroscience (145:963)||2007|
Gastric ulceration and expression of prolactin receptor in the brain in Hatano high- and low-avoidance rats.
MA1-610 was used in immunohistochemistry to study the relationship between gastric ulcers and brain prolactin receptor levels in Hatano low- and high-avoidance rats following restraint stress
|Asai S,Ohta R,Fujikawa T,Sakai RR,Shirota M,Ogata M,Watanabe G,Taya K||Endocrine (30:161)||2006|
Synchronous selection of homing peptides for multiple tissues by in vivo phage display.
MA1-610 was used in immunohistochemistry to develop an extremely efficient method to simultaneously screen phage display libraries for peptides homing to various tissues
|Kolonin MG,Sun J,Do KA,Vidal CI,Ji Y,Baggerly KA,Pasqualini R,Arap W||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (20:979)||2006|
Quantitation of prolactin receptor mRNA in the maternal rat brain during pregnancy and lactation.
MA1-610 was used in immunohistochemistry to investigate the changes of prolactin receptor transcription in pregnant and lactational rat brain
|Augustine RA,Kokay IC,Andrews ZB,Ladyman SR,Grattan DR||Journal of molecular endocrinology (31:221)||2003|
Mechanisms for suckling-induced changes in expression of prolactin receptor in the hypothalamus of the lactating rat.
MA1-610 was used in immunohistochemistry to investigate the suckling-induced changes in prolactin receptor expression in rat hypothalamus
|Pi X,Voogt JL||Brain research (891:197)||2001|
Effect of suckling on prolactin receptor immunoreactivity in the hypothalamus of the rat.
MA1-610 was used in immunohistochemistry to study the effect of suckling on the immunohistochemical expression of the prolactin receptor in rat hypothalamus
|Pi X,Voogt JL||Neuroendocrinology (71:308)||2000|
Increased prolactin receptor immunoreactivity in the hypothalamus of lactating rats.
MA1-610 was used in immunohistochemistry to study the relationship between lactation and the expression of prolactin receptor in rat hypothalamus
|Pi XJ,Grattan DR||Journal of neuroendocrinology (11:693)||1999|
Prolactin (PRL) receptor gene expression in mouse adipose tissue: increases during lactation and in PRL-transgenic mice.
MA1-610 was used in immunoprecipitation to determine the expression and regulation of PRL receptor in mouse adipose tissue.
|Ling C,Hellgren G,Gebre-Medhin M,Dillner K,Wennbo H,Carlsson B,Billig H||Endocrinology (141:3564)||2000|
Prolactin-induced cell proliferation in PC12 cells depends on JNK but not ERK activation.
MA1-610 was used in immunoprecipitation to investigate the role of JNK activation during the prolactin-induced cell proliferation.
|Cheng Y,Zhizhin I,Perlman RL,Mangoura D||The Journal of biological chemistry (275:23326)||2000|
Mitogenic and binding properties of monoclonal antibodies to the prolactin receptor in Nb2 rat lymphoma cells. Selective enhancement by anti-mouse IgG.
MA1-610 was used in blocking/activating experiment to perform functional analysis of monoclonal antibodies against prolactin receptor in Nb3 rat lymphoma cells
|Elberg G,Kelly PA,Djiane J,Binder L,Gertler A||The Journal of biological chemistry (265:14770)||1990|