MA1-611 detects prolactin (PRL) receptor in rat and human tissues. This antibody does not cross-react with PRL receptors from other species nor with growth hormone (GH) receptor.
MA1-611 has been successfully used in Western blot, IF, immunohistochemistry (4% PFA fixed tissue), and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells with MA1-611 yields a staining pattern consistent with cytoplasmic vesicular staining. MA1-611 has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.
The MA1-611 immunogen is purified rat liver PRL receptor.
Reconstitute with 100 µL PBS.
Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hPRL receptor; Lactogen receptor; Prl R; PRL-R; Prolactin receptor; secreted prolactin binding protein
Gene Aliases: HPRL; hPRLrI; MFAB; PRLR; RATPRLR