Invitrogen

Prolactin Receptor Monoclonal Antibody (U5)

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Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Prolactin Receptor using Prolactin Receptor Monoclonal Antibody (U5) (Product # MA1-610) shows staining in MCF-7 Cells. Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor (Product # MA1-610) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Prolactin Receptor Antibody in Immunohistochemistry (IHC) — Mammary gland tissue from lactating light horse mare (equine) was incubated in MA1-610 anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23°C and for 24 hours at 4°C. Second antibody was biotinylated goat anti-mouse IgG at 23°C for 1 hour. Development using avidin-biotin complex 23°C for 1 hour plus 3, 3-diaminobenzadine for 15 min and counterstained with haematoxylin. Notes: Antigen retrieval was facilitated using steaming at 95°C for 20 min in Trizma® buffer (pH 10.0). Blocking endogenous peroxidase performed with 0.3% H2O2 and MeOH (20 min at 23°C). To decrease non-specific staining, tissue was pre-incubated in normal goat serum for 30 min at 23°C. Data courtesy of the Innovators Program.

Prolactin Receptor Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — FIGURE 5 Immunocytochemical detection of PRL receptors in cumulus cells after 20 h maturation of bovine cumulus-enclosed oocytes. Specific localizations were detected by MA1-610 antibody and the red 3-amino-9-ethylcarbazole (AEC) chromophore. Nuclei were counterstained with hematoxylin. (A) Positive staining. Black arrows indicate PRL receptor-specific immunoreaction. (B) Negative control performed by omitting the primary antibody. Original magnification: x 400.

Product Details

MA1-610

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 12 publications 12 publications
Immunohistochemistry (IHC)	-	View 11 publications 11 publications
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	5 µg/mL	View 6 publications 6 publications
Flow Cytometry (Flow)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	View 2 publications 2 publications
Neutralization (Neu)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Horse, Human, Pig, Rabbit, Rat
Published species	Baboon, Bovine, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	U5
Immunogen	Purified rat liver PRL receptor.
Conjugate	Unconjugated
Form	Lyophilized
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2171291

Product Specific Information

MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues.

MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor.

The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor.

Reconstitute with 100 µL PBS.

Target Information

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prolactin Expression in the Baboon (Papio hamadryas) Eye.

Animals : an open access journal from MDPI

Garza-Rodríguez ML,Rodríguez-Sanchez IP,González-Álvarez R,Luna M,Burciaga-Flores CH,Alcorta-Nuñez F,Solis-Coronado O,Bautista de Lucio VM,Ramírez-Correa GA,Vidal-Gutiérrez O,Pérez-Ibave DC

MA1-610 was used in Immunohistochemistry to determine the expression of PRL and PRL receptor (PRLR) (mRNAs and proteins) in adult and fetal baboon (Papio hamadryas) ocular tissues.

Sat Sep 03 00:00:00 EDT 2022

Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways.

Frontiers in genetics

Lebedeva IY,Singina GN,Lopukhov AV,Shedova EN,Zinovieva NA

Published figure using Prolactin Receptor monoclonal antibody (Product # MA1-610) in Immunocytochemistry

Fri Sep 18 00:00:00 EDT 2015

Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways.

Frontiers in genetics

Lebedeva IY,Singina GN,Lopukhov AV,Shedova EN,Zinovieva NA

Published figure using Prolactin Receptor monoclonal antibody (Product # MA1-610) in Immunocytochemistry

Fri Sep 18 00:00:00 EDT 2015

Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways.

Frontiers in genetics

Lebedeva IY,Singina GN,Lopukhov AV,Shedova EN,Zinovieva NA

Published figure using Prolactin Receptor monoclonal antibody (Product # MA1-610) in Immunocytochemistry

Fri Sep 18 00:00:00 EDT 2015

Prolactin affects bovine oocytes through direct and cumulus-mediated pathways.

Theriogenology

Lebedeva IY,Singina GN,Volkova NA,Vejlsted M,Zinovieva NA,Schmidt M

MA1-610 was used in immunocytochemistry to characterize pathways of PRL action on bovine oocytes

Sat Nov 01 00:00:00 EDT 2014

Mechanisms of transient signaling via short and long prolactin receptor isoforms in female and male sensory neurons.

The Journal of biological chemistry

Belugin S,Diogenes AR,Patil MJ,Ginsburg E,Henry MA,Akopian AN

MA1-610 was used in immunohistochemistry and western blot to study transient prolactin signaling mediated by the short and the long forms of the prolactin receptor in sensory neurons of male and female rats

Fri Nov 29 00:00:00 EST 2013

Prolactin reduces the damaging effects of excitotoxicity in the dorsal hippocampus of the female rat independently of ovarian hormones.

Neuroscience

Tejadilla D,Cerbón M,Morales T

MA1-610 was used in immunohistochemistry to study the protective effect of prolactin against dorsal hippocampal excitotoxicity in female rats

Wed Sep 01 00:00:00 EDT 2010

Body reserves and ovarian performance in primiparous lactating rabbit does submitted to early weaning as a strategy to decrease energy deficit.

Animal reproduction science

Sakr OG,García-García RM,Arias-Alvarez M,Millán P,Lorenzo PL,Rebollar PG

MA1-610 was used in immunohistochemistry to study the effect of early weaning on metabolism and ovarian function in rabbits.

Wed Sep 01 00:00:00 EDT 2010

Prolactin does not affect human platelet aggregation or secretion.

Thrombosis and haemostasis

Reuwer AQ,Nieuwland R,Fernandez I,Goffin V,van Tiel CM,Schaap MC,Berckmans RJ,Kastelein JJ,Twickler MT

MA1-610 was used in western blot to study the lack of effect of prolactin on human platelet function

Mon Jun 01 00:00:00 EDT 2009

Prolactin activates mitogen-activated protein kinase signaling and corticotropin releasing hormone transcription in rat hypothalamic neurons.

Endocrinology

Blume A,Torner L,Liu Y,Subburaju S,Aguilera G,Neumann ID

MA1-610 was used in western blot to study the effect of prolactin on MAP kinase signaling and CRH transcription in rat hypothalamic neurons.

Wed Apr 01 00:00:00 EDT 2009

Prolactin prevents chronic stress-induced decrease of adult hippocampal neurogenesis and promotes neuronal fate.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Torner L,Karg S,Blume A,Kandasamy M,Kuhn HG,Winkler J,Aigner L,Neumann ID

MA1-610 was used in western blot to study the role of prolactin in the neurogenesis in hippocampal dentate gyrus.

Wed Feb 11 00:00:00 EST 2009

Prolactin is involved in glial responses following a focal injury to the juvenile rat brain.

Neuroscience

Möderscheim TA,Gorba T,Pathipati P,Kokay IC,Grattan DR,Williams CE,Scheepens A

MA1-610 was used in immunohistochemistry to study the role of prolactin in glial responses following a focal injury to the juvenile rat brain

Fri Mar 30 00:00:00 EDT 2007

Overexpression of the mTOR alpha4 phosphoprotein activates protein phosphatase 2A and increases Stat1alpha binding to PIAS1.

Molecular and cellular endocrinology

Nien WL,Dauphinee SM,Moffat LD,Too CK

MA1-610 was used in western blot to study the activation of protein phosphatase 2A by the mTOR alpha4 phosphoprotein

Mon Jan 15 00:00:00 EST 2007

Gastric ulceration and expression of prolactin receptor in the brain in Hatano high- and low-avoidance rats.

Endocrine

Asai S,Ohta R,Fujikawa T,Sakai RR,Shirota M,Ogata M,Watanabe G,Taya K

MA1-610 was used in immunohistochemistry to study the relationship between gastric ulcers and brain prolactin receptor levels in Hatano low- and high-avoidance rats following restraint stress

Sun Oct 01 00:00:00 EDT 2006

A novel estradiol/estrogen receptor alpha-dependent transcriptional mechanism controls expression of the human prolactin receptor.

The Journal of biological chemistry

Dong J,Tsai-Morris CH,Dufau ML

MA1-610 was used in western blot to study the mechanism for estradiol-induced human prolactin receptor expression.

Fri Jul 07 00:00:00 EDT 2006

ERK3 associates with MAP2 and is involved in glucose-induced insulin secretion.

Molecular and cellular endocrinology

Anhê GF,Torrão AS,Nogueira TC,Caperuto LC,Amaral ME,Medina MC,Azevedo-Martins AK,Carpinelli AR,Carvalho CR,Curi R,Boschero AC,Bordin S

MA1-610 was used in western blot to study the role of ERK3 and MAP2 in glucose-induced insulin secretion

Wed Jun 07 00:00:00 EDT 2006

Synchronous selection of homing peptides for multiple tissues by in vivo phage display.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kolonin MG,Sun J,Do KA,Vidal CI,Ji Y,Baggerly KA,Pasqualini R,Arap W

MA1-610 was used in immunohistochemistry to develop an extremely efficient method to simultaneously screen phage display libraries for peptides homing to various tissues

Mon May 01 00:00:00 EDT 2006

Prolactin-regulated tyrosine hydroxylase activity and messenger ribonucleic acid expression in mediobasal hypothalamic cultures: the differential role of specific protein kinases.

Endocrinology

Ma FY,Grattan DR,Goffin V,Bunn SJ

MA1-610 was used in immunocytochemistry to investigate the different roles of protein kinases in prolactin-regulated tyrosine hydroxylase activity.

Sat Jan 01 00:00:00 EST 2005

Quantitation of prolactin receptor mRNA in the maternal rat brain during pregnancy and lactation.

Journal of molecular endocrinology

Augustine RA,Kokay IC,Andrews ZB,Ladyman SR,Grattan DR

MA1-610 was used in immunohistochemistry to investigate the changes of prolactin receptor transcription in pregnant and lactational rat brain

Fri Aug 01 00:00:00 EDT 2003

Response of Diamond-Blackfan anemia to metoclopramide: evidence for a role for prolactin in erythropoiesis.

Blood

Abkowitz JL,Schaison G,Boulad F,Brown DL,Buchanan GR,Johnson CA,Murray JC,Sabo KM

MA1-610 was used in immunocytochemistry to study the role of prolactin during the erythropoiesis

Tue Oct 15 00:00:00 EDT 2002

Src family kinases are required for prolactin induction of cell proliferation.

Molecular biology of the cell

Fresno Vara JA,Cáceres MA,Silva A,Martín-Pérez J

MA1-610 was used in western blot to study the role of Src family of tyrosine kinases (SFK) on PRL-induced cell proliferation

Sun Jul 01 00:00:00 EDT 2001

Mechanisms for suckling-induced changes in expression of prolactin receptor in the hypothalamus of the lactating rat.

Brain research

Pi X,Voogt JL

MA1-610 was used in immunohistochemistry to investigate the suckling-induced changes in prolactin receptor expression in rat hypothalamus

Fri Feb 09 00:00:00 EST 2001

Constitutive tyrosine phosphorylation of ErbB-2 via Jak2 by autocrine secretion of prolactin in human breast cancer.

The Journal of biological chemistry

Yamauchi T,Yamauchi N,Ueki K,Sugiyama T,Waki H,Miki H,Tobe K,Matsuda S,Tsushima T,Yamamoto T,Fujita T,Taketani Y,Fukayama M,Kimura S,Yazaki Y,Nagai R,Kadowaki T

MA1-610 was used in immunocytochemistry to investigate the mechanism for constitutive tyrosine-phosphorylation of ErbB2 in human breast cancer

Fri Oct 27 00:00:00 EDT 2000

Prolactin (PRL) receptor gene expression in mouse adipose tissue: increases during lactation and in PRL-transgenic mice.

Endocrinology

Ling C,Hellgren G,Gebre-Medhin M,Dillner K,Wennbo H,Carlsson B,Billig H

MA1-610 was used in immunoprecipitation to determine the expression and regulation of PRL receptor in mouse adipose tissue.

Sun Oct 01 00:00:00 EDT 2000

Prolactin-induced cell proliferation in PC12 cells depends on JNK but not ERK activation.

The Journal of biological chemistry

Cheng Y,Zhizhin I,Perlman RL,Mangoura D

MA1-610 was used in immunoprecipitation to investigate the role of JNK activation during the prolactin-induced cell proliferation.

Fri Jul 28 00:00:00 EDT 2000

Effect of suckling on prolactin receptor immunoreactivity in the hypothalamus of the rat.

Neuroendocrinology

Pi X,Voogt JL

MA1-610 was used in immunohistochemistry to study the effect of suckling on the immunohistochemical expression of the prolactin receptor in rat hypothalamus

Mon May 01 00:00:00 EDT 2000

Suppression of nerve growth factor Trk receptors and prolactin receptors by endocannabinoids leads to inhibition of human breast and prostate cancer cell proliferation.

Endocrinology

Melck D,De Petrocellis L,Orlando P,Bisogno T,Laezza C,Bifulco M,Di Marzo V

MA1-610 was used in western blot to investigate whether and through what mechanism anandamide and 2-AG inhibit the nerve growth factor-induced proliferation of HBCCs and the PRL-induced proliferation of human prostate cancer cells.

Sat Jan 01 00:00:00 EST 2000

Increased prolactin receptor immunoreactivity in the hypothalamus of lactating rats.

Journal of neuroendocrinology

Pi XJ,Grattan DR

MA1-610 was used in immunohistochemistry to study the relationship between lactation and the expression of prolactin receptor in rat hypothalamus

Wed Sep 01 00:00:00 EDT 1999

Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation.

Journal of cell science

Perrot-Applanat M,Gualillo O,Buteau H,Edery M,Kelly PA

MA1-610 was used in immunocytochemistry to investigate the mechanism for the internalization of prolactin and its receptor

Thu May 01 00:00:00 EDT 1997

Null mutation of the prolactin receptor gene produces multiple reproductive defects in the mouse.

Genes & development

Ormandy CJ,Camus A,Barra J,Damotte D,Lucas B,Buteau H,Edery M,Brousse N,Babinet C,Binart N,Kelly PA

MA1-610 was used in western blot to investigate the consequences of null mutation of the prolactin receptor with respect to reproduction in a mouse model

Wed Jan 15 00:00:00 EST 1997

Mutational analysis of the ligand-binding domain of the prolactin receptor.

The Journal of biological chemistry

Rozakis-Adcock M,Kelly PA

MA1-610 was used in western blot to perform mutational analysis of prolactin receptor in order to investigate the contributions of different domains to hormone binding

Thu Sep 05 00:00:00 EDT 1991

Mitogenic and binding properties of monoclonal antibodies to the prolactin receptor in Nb2 rat lymphoma cells. Selective enhancement by anti-mouse IgG.

The Journal of biological chemistry

Elberg G,Kelly PA,Djiane J,Binder L,Gertler A

MA1-610 was used in blocking/activating experiment to perform functional analysis of monoclonal antibodies against prolactin receptor in Nb3 rat lymphoma cells

Wed Sep 05 00:00:00 EDT 1990

Purification and protein sequence analysis of rat liver prolactin receptor.

The Journal of biological chemistry

Okamura H,Raguet S,Bell A,Gagnon J,Kelly PA

MA1-610 was used in western blot to characterize prolactin receptors in rat liver

Wed Apr 05 00:00:00 EDT 1989

Bioinformatics

Protein Aliases: cytokine receptor; hPRL receptor; Lactogen receptor; Prl R; PRL-R; Prolactin receptor; prolactin receptor 2 precursor; prolactin receptor short isoform; secreted prolactin binding protein

Gene Aliases: HPRL; hPRLrI; MFAB; pPRLR-SF; PRLR; RATPRLR

UniProt ID: (Rabbit) P14787, (Pig) Q6JTA8, (Human) P16471, (Rat) P05710

Entrez Gene ID: (Rabbit) 100009046, (Pig) 414916, (Human) 5618, (Horse) 100053793, (Rat) 24684

Molecular Function: transmembrane signal receptor