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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Prolactin Receptor Antibodies

          Invitrogen

          Prolactin Receptor Monoclonal Antibody (U5)

          Advanced Verification
          4 Published Figures
          36 References
          View all (36) Prolactin Receptor antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Prolactin Receptor Monoclonal Antibody (U5)

          • Antibody Testing Data (3)
          • Published Figures (4)
          • Advanced Verification (1)
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Prolactin Receptor Antibody (MA1-610) in IHC

          Mammary gland tissue from lactating light horse mare (equine) was incubated in MA1-610 anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23°C and for 24 hours at 4°C. Second antibody was biotinylated goat anti-mouse IgG at 23°C for 1 hour. Development using avidin-biotin complex 23°C for 1 hour plus 3, 3-diaminobenzadine for 15 min and counterstained with haematoxylin. Notes: Antigen ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody

          Product Details

          MA1-610

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 15 publications 15 publications

          Immunohistochemistry (IHC)

          -
          View 11 publications 11 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          View 2 publications 2 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Horse, Human, Mouse, Pig, Rabbit, Rat

          Published species

          Baboon, Bovine, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          U5

          Immunogen

          Purified rat liver PRL receptor.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2171291

          Product Specific Information

          MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues.

          MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor.

          The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor.

          Reconstitute with 100 µL PBS.

          Target Information

          Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cytokine receptor; hPRL receptor; Lactogen receptor; Prl R; PRL-R; Prolactin receptor; prolactin receptor 2 precursor; prolactin receptor related sequence 1; prolactin receptor short isoform; secreted prolactin binding protein

          View more View less

          Gene Aliases: AI987712; HPRL; hPRLrI; MFAB; pPRLR-SF; Pr-1; Pr-3; PRLR; Prlr-rs1; RATPRLR

          View more View less

          UniProt ID: (Rabbit) P14787, (Pig) Q6JTA8, (Human) P16471, (Rat) P05710, (Mouse) Q08501

          View more View less

          Entrez Gene ID: (Rabbit) 100009046, (Pig) 414916, (Human) 5618, (Horse) 100053793, (Rat) 24684, (Mouse) 19116

          View more View less

          Function(s)
          metal ion binding prolactin receptor activity protein binding peptide hormone binding protein homodimerization activity ornithine decarboxylase activator activity cytokine receptor activity protein kinase binding transmembrane signal receptor
          Process(es)
          prolactin signaling pathway oncostatin-M-mediated signaling pathway leukemia inhibitory factor signaling pathway steroid biosynthetic process cell surface receptor signaling pathway activation of transmembrane receptor protein tyrosine kinase activity embryo implantation lactation regulation of cell adhesion regulation of epithelial cell differentiation T cell activation activation of JAK2 kinase activity negative regulation of apoptotic process JAK-STAT cascade involved in growth hormone signaling pathway mammary gland epithelial cell differentiation prostate gland growth mammary gland alveolus development JAK-STAT cascade cytokine-mediated signaling pathway mammary gland epithelium development
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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