Immunofluorescence analysis of Prostate Specific Acid Phosphatase Antibody (PASE/4LJ) was performed using 70% confluent log phase LNCaP cells. The cells were fixed with methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Prostate Specific Acid Phosphatase Antibody (PASE/4LJ) Mouse Monoclonal Antibody (MA513078) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product# PA516891, 1ug/ml) followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (A11037) (Panel c: red). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified prostatic acid phosphatase (PSAP) from human seminal plasma|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA5-13078 targets Prostate Specific Acid Phosphatase in IF and IHC (P) applications and shows reactivity with Human samples.
The MA5-13078 immunogen is purified prostatic acid phosphatase (PSAP) from human seminal plasma.
Prostatic acid phosphatase (PSAP) is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thyroid transcription factor-1 may be expressed in ductal adenocarcinoma of the prostate:a potential pitfall.
MA5-13078 was used in immunohistochemistry to study the expression of thyroid transcription factor-1 in ductal adenocarcinoma of the prostate
|Lim TK,Teo C,Giron DM,Chong YL,Cheng C,Tan PH||Journal of clinical pathology (60:941)||2007|
Metastasizing seminal vesicle adenocarcinoma in a Wistar rat.
MA5-13078 was used in immunohistochemistry to report on a case of metastasizing seminal vesicle adenocarcinoma in a Wistar rat
|Teredesai A,Wöhrmann T||Journal of veterinary medicine. A, Physiology, pathology, clinical medicine (52:131)||2005|