|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human recombinant proteasome 19S subunit S10B.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-971 detects proteasome 19S subunit S10B from human cells.
PA1-971 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 42 kDa protein representing proteasome 19S subunit S10B from HeLa cell lysate. This antibody detects, to a lesser extent, an ~30 kDa protein which could correspond to subunit degradation product.
PA1-971 antigen is recombinant human proteasome 19S subunit S10B.
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman and quote;s syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 26S proteasome non-ATPase regulatory subunit 2; 26S proteasome regulatory subunit RPN1; 26S proteasome regulatory subunit S2; 26S proteasome subunit p97; 55.11 protein; MGC14274; P97; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; Protein 55.11; Rpn1; TNFR-associated protein 2; TRAP2; Tumor necrosis factor type 1 receptor-associated protein 2
Gene Aliases: P97; PSMD2; RPN1; S2; TRAP2
UniProt ID: (Human) Q13200
Entrez Gene ID: (Human) 5708
Molecular Function: enzyme modulator
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.