|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1 - 3 µg/ml|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat , Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-1962 detects proteasome 20S subunit alpha 5 from human, rat, mouse and hamster cell lysates.
PA1-1962 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 26.5 kDa protein representing proteasome 20S subunit alpha 5 from human, rat, mouse, and hamster cell extracts.
PA1-1962 immunogen is a synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5. PA1-1962 immunizing peptide (Cat. # PEP-210) is available for use in neutralization and control experiments.
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman and quote;s syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: epididymis tissue sperm binding protein Li 11n; macropain subunit zeta; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome alpha 5 subunit; proteasome component 5; Proteasome subunit alpha type-5; proteasome subunit alpha type-5-like protein; proteasome subunit zeta; Proteasome zeta chain
Gene Aliases: PSC5; PSMA5; ZETA
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.