|Tested species reactivity||Bovine, Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C E(263) I E V L E E T V Q T M D T S(NH2)(277)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-979 detects proteasome 20S Z (also known as the beta 2 subunit) from purified bovine and human 26S proteasome samples.
PA1-979 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~29 kDa protein representing proteasome 20S Z from purified human 26S proteasome.
The PA1-979 immunizing peptides correspond to amino acid residues 263-277 from human proteasome 20S Z protein. This sequence is completely conserved in mouse and 97% conserved in rat protein. PA1-979 immunizing peptide (Cat. # PEP-175) is available for use in neutralization and control procedures.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Persistent hijacking of brain proteasomes in HIV-associated dementia.
PA1-979 was used in western blot to study the effect of HIV infection on brain proteasomes and its role in dementia
|Nguyen TP,Soukup VM,Gelman BB||The American journal of pathology (176:893)||2010|
Neuropathology and pathogenesis of encephalitis following amyloid-beta immunization in Alzheimer's disease.
PA1-979 was used in immunohistochemistry to investigate the cause of meningoencephalitis after immunization with amyloid-beta peptide in Alzheimer disease
|Ferrer I,Boada Rovira M,Sánchez Guerra ML,Rey MJ,Costa-Jussá F||Brain pathology (Zurich, Switzerland) (14:11)||2004|