|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 238 of Human Proteasome 20S alpha 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
PA5-28030 targets Proteasome 20S alpha 1 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-28030 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 238 of Human Proteasome 20S alpha 1.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
1; 20S proteasome subunit C2; 30 kDa prosomal protein; alpha-type; epididymis secretory protein Li 275; HC2; macropain subunit C2; macropain subunit nu; multicatalytic endopeptidase complex subunit C2; NU; p; PROS-30; PROS30; proteasome (prosome, macropain) subunit, alpha type, 1; proteasome component C2; proteasome nu chain; proteasome subunit; proteasome subunit alpha type-1; proteasome subunit nu; proteasome subunit, alpha-type, 1; protein P30-33K; testicular tissue protein Li 150
alpha-type; C2; HC2; HEL-S-275; NU; Pros-30; PROS30; PSC2; PSMA1