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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) A S L L K V D Q E V K L K(14) C of human proteasome activator 11S REG gamma.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-961 detects recombinant human proteasome activator 11S REG gamma subunit.
PA1-961 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 28 kDa protein representing recombinant human 11S REG gamma.
The PA1-961 immunogen is a synthetic peptide corresponding to residues M(1) A S L L K V D Q E V K L K(14) C of human proteasome activator 11S REG gamma. The PA1-961 immunizing peptide (Cat. # PEP-091) is available for use in neutralization and control experiments.
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman"e;s syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
11S regulator complex gamma subunit; 11S regulator complex subunit gamma; activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; prosome macropain; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); proteasome activator 28 subunit gamma; proteasome activator 28-gamma; Proteasome Activator11S; REG gamma-3
HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; PSME3; REG-GAMMA