Immunofluorescent analysis of Proteasome inhibitor PI31 subunit showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Proteasome inhibitor PI31 subunit polyclonal antibody (Product # PA5-21603) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 209 and 271 of PSMF1 (Uniprot ID#Q92530)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 40% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21603 targets Proteasome inhibitor PI31 subunit in WB applications and shows reactivity with Human samples.
The PA5-21603 immunogen is a synthetic peptide corresponding to a region within amino acids 209 and 271 of PSMF1 (Uniprot ID#Q92530).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.