HYB 237-01-02 has specificity for human protein C.
HYB 237-01-02 has been successfully used in immunohistochemistry, ELISA, and Western blot procedures. HYB 237-01-02 reacts strongly with protein C. Strong reaction is seen in catching ELISA in combination with a biotin labeled antigen against protein C, but only when using a tris-buffer containing 2,5 mM CaCl2. In Western blotting after SDS-PAGE, HYB 237-01-02 reacts with protein C in non-reduced form only.
The HYB 237-01-02 immunogen is protein C isolated from human plasma.
Epitope specificity differs from that of HYB 237-05-02.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Protein C is a vitamin K-dependent plasma glycoprotein that is cleaved to its activated form by the thrombin-thrombomodulin complex. Protein C in its activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/mL because of the very fast turnover, the proenzyme concentration is approximately 3 µg/mL. Mutations in the Protein C gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activated blood coagulation factor XIV; Activation peptide; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; prepro-protein C; protein C (inactivator of coagulation factors Va and VIIIa); type I protein C; Vitamin K dependent protein C precursor; Vitamin K-dependent protein C; Vitamin K-dependent protein C heavy chain; Vitamin K-dependent protein C light chain
Gene Aliases: APC; PC; PROC; PROC1; THPH3; THPH4
UniProt ID: (Human) P04070
Entrez Gene ID: (Human) 5624