|Tested species reactivity||Virus|
|Published species reactivity||Rat, Virus, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified pseudorabies virus-Bartha.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1.0 µg/ml|
|Western Blot (WB)||0.2 - 1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-081 detects pseudorabies virus (PRV).
PA1-081 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects proteins in the range of 80-115 kDa representing various PRV proteins. Immunohistochemical staining of PRV in rat hypothalamus from animals injected with PRV into the vitreous body of the eye results in intense staining of neurons in the superchiasmatic nucleus.
The PA1-081 immunogen is purified PRV-Bartha.
Herpes simplex virus (HSV), varicella-zoster virus (VZV) and pseudorabies virus (PRV) comprise a family of neurotropic viruses known as alphaherpesviruses. PRV is primarily a disease of swine which serve as a reservoir for the virus and the principal source of natural infection for a diverse range of secondary hosts, including cattle, sheep, goats, dogs, cats, and many feral species. Humans and apes are refractory to PRV infection. PRV infects the epithelium of the host where it then spreads to axons of the peripheral nervous system (PNS). The virus opportunistically avails itself of the neuronal retrograde transport pathway which can facilitate transfer to the brain. This unique aspect of PRV allows it to be used as a powerful research tool to study neural connectivity and trace neuronal circuitry in the peripheral (PNS) and central nervous system (CNS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Brainstem metabotropic glutamate receptors reduce food intake and activate dorsal pontine and medullar structures after peripheral bacterial lipopolysaccharide administration.
PA1-081 was used in immunohistochemistry - free floating to assess the activation of dorsal pontine and medullar structures and a reduction of food intake after peripheral bacterial lipopolysaccharide administration through brainstem metabotropic glutama
|Chaskiel L,Paul F,Gerstberger R,Hübschle T,Konsman JP||Neuropharmacology (107:146)||2016|
Blocking ESCRT-mediated envelopment inhibits microtubule-dependent trafficking of alphaherpesviruses in vitro.
PA1-081 was used in western blot to study the role of ESCRT in microtubule-mediated trafficking of naked and enveloped alpha herpesvirus capsids
|Kharkwal H,Smith CG,Wilson DW||Journal of virology (88:14467)||2014|
Bilateral bulbospinal projections to pudendal motoneuron circuitry after chronic spinal cord hemisection injury as revealed by transsynaptic tracing with pseudorabies virus.
PA1-081 was used in immunohistochemistry to study the bilateral bulbospinal pathway and its role in functional recovery after after chronic spinal cord hemisection injury
|Johnson RD,Chadha HK,Dugan VP,Gupta DS,Ferrero SL,Hubscher CH||Journal of neurotrauma (28:595)||2011|
Hypothalamic neurons innervating fat tissue in the pig express leptin receptor immunoreactivity.
PA1-081 was used in immunohistochemistry to investigate the expression of leptin receptors in fat tissues innervated with hypothalamic neurons
|Czaja K,Barb CR,Kraeling RR||Neuroscience letters (425:6)||2007|
Identification of sympathetic premotor neurons in medullary raphe regions mediating fever and other thermoregulatory functions.
PA1-081 was used in immunohistochemistry to investigate the role of sympathetic premotor neurons in medullary raphe regions.
|Nakamura K,Matsumura K,Hübschle T,Nakamura Y,Hioki H,Fujiyama F,Boldogköi Z,König M,Thiel HJ,Gerstberger R,Kobayashi S,Kaneko T||The Journal of neuroscience : the official journal of the Society for Neuroscience (24:5370)||2004|
Definition of neuronal circuitry controlling the activity of phrenic and abdominal motoneurons in the ferret using recombinant strains of pseudorabies virus.
PA1-081 was used in immunohistochemistry to investigate how phrenic and abdominal motoneuron activity is controlled
|Billig I,Foris JM,Enquist LW,Card JP,Yates BJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (20:7446)||2000|
The role of virion membrane protein endocytosis in the herpesvirus life cycle.
PA1-081 was used in immunocytochemistry to investigate the importance of the endocytosis of virus membrane proteins during the pseudorabies virus life cycle
|Brideau AD,Enquist LW,Tirabassi RS||Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (17:69)||2000|