Immunohistochemical analysis of Psoriasin in formalin-fixed, paraffin-embedded human breast tissue using a Psoriasin monoclonal antibody (Product # MA1-91555) at a dilution of 1:200 using a peroxidase-conjugate and DAB chromogen. Antigen retrieval was performed with a citrate buffer, pH 6.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full length protein (Human).|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91555 detects Psoriasin from human samples.
MA1-91555 has been successfully used in immunohistochemistry and Western blot applications, where a band of ~11 kDa is detected. Confluent MCF10A cells can be used as a positive control for this antibody.
The MA1-91555 immunogen is recombinant human full length protein.
Store at 4°C short term. For long term storage, aliquot and store at -20°C or below, avoiding freeze-thaw cycles.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.