Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue stained with a Psoriasin/HID5/S100A7 monoclonal antibody (Product # MA5-16199) at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with citrate buffer, pH 6.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant psoriasin/HID-5 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: MCF10A, MCF-7.
Psoriasin/HID-5 was originally identified as an up-regulated protein in psoriatic keratinocytes (1). Subsequently, this protein was found to have elevated expression in abnormally differentiating primary keratinocytes and in various carcinomas, including ductal carcinoma in situ (2-3). Psoriasin/HID-5 may play a role in the establishment or maintenance of these pathologies, and may serve as a diagnostic marker of particular tumor types or abnormally proliferative epithelia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.