Immunofluorescent analysis of Pygopus 2 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Pygopus 2 polyclonal antibody (Product # PA5-30828) diluted at 1:200. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 190 and 385 of Human Pygopus 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30828 targets Pygopus 2 in WB applications and shows reactivity with Human samples.
The PA5-30828 immunogen is recombinant protein fragment corresponding to a region within amino acids 190 and 385 of Human Pygopus 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.