Western blot analysis of Pyruvate Kinase (liver, RBC) using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate (C) 30 µg K562 whole cell lysate (D) 30 µg THP-1 whole cell lysate and E) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Pyruvate Kinase (liver, RBC) polyclonal antibody (Product # PA5-28677) at a dilution of 1:1000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 254 and 574 of Human Pyruvate Kinase|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28677 targets Pyruvate Kinase (liver/RBC) in WB applications and shows reactivity with Human, Mouse and rat samples.
The PA5-28677 immunogen is recombinant fragment corresponding to a region within amino acids 254 and 574 of Human Pyruvate Kinase.
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
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