Western blot analysis of Pyruvate Kinase (liver/RBC) using A) 30 µg 293T whole cell lysate and B) 5 µg whole cell lysate of human PKLR-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Pyruvate Kinase (liver/RBC) polyclonal antibody (Product # PA5-34734) at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 301 of Pyruvate Kinase (liver/RBC) (Uniprot ID#P30613)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34734 targets Pyruvate Kinase (liver/RBC) in WB applications and shows reactivity with Human, Mouse, and Rat samples.
The PA5-34734 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 301 of Pyruvate Kinase (liver/RBC) (Uniprot ID#P30613).
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
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