Immunofluorescent analysis of Pyruvate Kinase(liver, RBC) showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in -20°C 100% MeOH for 5 min and stained using a Pyruvate Kinase(liver, RBC) polyclonal antibody (Product # PA5-22178) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 260 of Pyruvate Kinase (liver/RBC) (Uniprot ID#P30613)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22178 targets Pyruvate Kinase(liver/RBC) in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-22178 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 260 of Pyruvate Kinase (liver/RBC) (Uniprot ID#P30613).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.