|Flow Cytometry (Flow)||0.5 µg/test|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The 69H1-9-9 monoclonal antibody reacts with the mouse Qa-2 molecule, a GPI-linked MHC class Ibeta surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level is different depending on the laboratory mouse strain.
Applications Reported: The 69H1-9-9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 69H1-9-9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Qa-2 is a GPI-linked MHC class I beta surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level varies between laboratory mouse strains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene Aliases: H2-Qa2; Ped; Qa-2
Entrez Gene ID: (Mouse) 111458
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