Immunofluorescent analysis of RAB4 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAB4 polyclonal antibody (Product # PA3-912) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). RAB4 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified, recombinant human Rab4 protein.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-912 detects Rab4 from human and mouse samples. This antibody does not detect other Rab isoforms (Rab1, Rab3, Rab5, or Rab6).
PA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~24 kDa band representing Rab4 from transfected HeLa cells.
The PA3-912 immunogen is purified recombinant human Rab4 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.