Invitrogen

RAB7B Recombinant Rabbit Monoclonal Antibody (SN202-03)

Product Image Gallery

RAB7B Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of RAB7B in HepG2 cells using a RAB7B Monoclonal antibody (Product # MA5-32340) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

RAB7B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of RAB7B of paraffin-embedded Mouse skeletal muscle tissue using a RAB7B Monoclonal antibody (Product #MA5-32340). Counter stained with hematoxylin.

RAB7B Antibody in Western Blot (WB) — Western blot analysis of RAB7B in different lysates using a Monoclonal antibody (Product #MA5-32340) at a dilution of 1:1,000. Positive control: Lane 1: A431, Lane 2: C2C12.

RAB7B Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of RAB7B in K562 cells using a RAB7B Monoclonal Antibody (Product # MA5-32340) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:50-1:100

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

1:500-1:2,000

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN202-03

Immunogen

Synthetic peptide within C-terminal human RAB7

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809621

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.