Invitrogen

RAB7A Recombinant Rabbit Monoclonal Antibody (SN202-03)

Product Image Gallery

RAB7A Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of RAB7A in HepG2 cells using a RAB7A Monoclonal antibody (Product # MA5-32340) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

RAB7A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of RAB7A of paraffin-embedded Mouse skeletal muscle tissue using a RAB7A Monoclonal antibody (Product #MA5-32340). Counter stained with hematoxylin.

RAB7A Antibody in Western Blot (WB) — Western blot analysis of RAB7A in different lysates using a Monoclonal antibody (Product #MA5-32340) at a dilution of 1:1,000. Positive control: Lane 1: A431, Lane 2: C2C12.

RAB7A Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of RAB7A in K562 cells using a RAB7A Monoclonal Antibody (Product # MA5-32340) at a dilution of 1:50, as seen in red compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32340

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	SN202-03
Immunogen	Synthetic peptide within Human RAB7 aa 158-207
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809621

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: rab-7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein BRL-RAS; Ras-related protein p23; Ras-related protein Rab-7a

Gene Aliases: PRO2706; RAB7; RAB7A

UniProt ID: (Human) P51149, (Mouse) P51150, (Rat) P09527

Entrez Gene ID: (Human) 7879, (Mouse) 19349, (Rat) 29448

Function(s)