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Invitrogen
RAB8A Recombinant Rabbit Monoclonal Antibody (JE64-79)
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FIGURE: 1 / 13
RAB8A Antibody (MA5-49140) in ICC/IF
Immunofluorescence of RAB8A was performed using HeLa WT (lane 1) and RAB8A KO (lane 2) cells that were labeled with CellTracker Green CMFDA (Product # C2925) or CellTracker™ Deep Red (Product # C34565), respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat ... View More
Product Details
MA5-49140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE64-79
Immunogen
Synthetic peptide within human rab8a aa 151-200/207.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Sequence Similarities: 100% Mouse/Rat
Tissue Specificity: Low tissue specificity
Positive Control: Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, L6 cell lysate
Subcellular location: Cell membrane, golgi apparatus, recycling endosome membrane, cytoplasm, centriole, cilium basal body, cilium axoneme, cilium, phagosome, phagosome membrane, midbody.
Target Information
The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell line NK14 derived transforming oncogene; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; RAB8A; ras-associated protein RAB8; Ras-related protein Rab-8A; unnamed protein product
Gene Aliases: AA409338; MEL; RAB8; RAB8A
UniProt ID: (Human) P61006, (Mouse) P55258, (Rat) P35280
Entrez Gene ID: (Human) 4218, (Mouse) 17274, (Rat) 117103
transport
intracellular protein transport
vesicle docking involved in exocytosis
small GTPase mediated signal transduction
axonogenesis
metabolic process
protein secretion
protein transport
synaptic vesicle exocytosis
regulation of exocytosis
cell projection organization
Rab protein signal transduction
cellular response to insulin stimulus
regulation of protein localization
cilium assembly
regulation of long-term neuronal synaptic plasticity
Golgi vesicle fusion to target membrane
regulation of protein transport
protein localization to plasma membrane
exocytosis
autophagy
Golgi organization
regulation of autophagy
endocytic recycling
cilium morphogenesis
protein localization to cilium
neurotransmitter receptor transport, endosome to postsynaptic membrane
neurotransmitter receptor transport to postsynaptic membrane
vesicle-mediated transport in synapse
glucose homeostasis
positive regulation of glucose import
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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