Immunofluorescent analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) (Product# MA3-067 ) shows staining in A2058 Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing RAB9 (Product# MA3-067 ) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Bovine, Dog, Hamster, Human, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant canine rab 9a.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-067 recognizes prenylated and non-prenylated rab 9 from canine, human, monkey, rat, hamster and bovine tissues. This antibody does not cross-react with other rab family members.
MA3-067 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~26 kDa protein representing rab 9 from K562 cell extract.
The MA3-067 antigen is recombinant canine rab 9a.
Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components.
It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Microvillus inclusion disease: loss of Myosin vb disrupts intracellular traffic and cell polarity.
MA3-067 was used in immunohistochemistry and western blot to study the role of defective intracellular trafficking and loss of cell polarity due to myosin Vb loss in the pathogenic mechanism of microvillus inclusion disease
|Thoeni CE,Vogel GF,Tancevski I,Geley S,Lechner S,Pfaller K,Hess MW,Müller T,Janecke AR,Avitzur Y,Muise A,Cutz E,Huber LA||Traffic (Copenhagen, Denmark) (15:22)||2014|
Increased mucosal nitric oxide production in ulcerative colitis is mediated in part by the enteroglial-derived S100B protein.
MA3-067 was used in immunohistochemistry to study the mechanism for the increased production of mucosal nitric oxide in ulcerative colitis
|Cirillo C,Sarnelli G,Esposito G,Grosso M,Petruzzelli R,Izzo P,Calì G,D'Armiento FP,Rocco A,Nardone G,Iuvone T,Steardo L,Cuomo R||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (21:1209)||2009|
Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain.
MA3-067 was used in flow cytometry to study the role of epidermal growth factor receptor in tumor cell proliferation
|Vernet E,Lundberg E,Friedman M,Rigamonti N,Klausing S,Nygren PA,Gräslund T||New biotechnology (25:417)||2009|
Coexpression of the melatonin receptor 1 and nestin in human breast cancer specimens.
MA3-067 was used in immunocytochemistry to study the expression and role of the melatonin receptor 1 and nestin in human breast cancer
|Rögelsperger O,Ekmekcioglu C,Jäger W,Klimpfinger M,Königsberg R,Krenbek D,Sellner F,Thalhammer T||Journal of pineal research (46:422)||2009|
Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.
MA3-067 was used in immunocytochemistry to investigate the mechanism of endocytosis of adherens junction proteins and tight junction proteins in T84 epithelial cells
|Ivanov AI,Nusrat A,Parkos CA||Molecular biology of the cell (15:176)||2004|
Nitric oxide-dependent processing of heparan sulfate in recycling S-nitrosylated glypican-1 takes place in caveolin-1-containing endosomes.
MA3-067 was used in immunocytochemistry to investigate the processing of heparan sulfate side chains from recycling glypican-1
|Cheng F,Mani K,van den Born J,Ding K,Belting M,Fransson LA||The Journal of biological chemistry (277:44431)||2002|
Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication.
MA3-067 was used in western blot to study the role of cellular Arf GTPases during poliovirus replication
|Belov GA,Habbersett C,Franco D,Ehrenfeld E||Journal of virology (81:9259)||2007|
Kaposi's sarcoma-associated herpesvirus K3 utilizes the ubiquitin-proteasome system in routing class major histocompatibility complexes to late endocytic compartments.
MA3-067 was used in western blot to study the function of Kaposi sarcoma-associated herpesvirus K3 in internalization and targeting of MHC class I complexes.
|Lorenzo ME,Jung JU,Ploegh HL||Journal of virology (76:5522)||2002|
Quantitative analysis of the interactions between prenyl Rab9, GDP dissociation inhibitor-alpha, and guanine nucleotides.
MA3-067 was used in western blot to investigate the interaction among prenyl Rab9, GDI and guanine nucleotides
|Shapiro AD,Pfeffer SR||The Journal of biological chemistry (270:11085)||1995|