Invitrogen

RAB9 Monoclonal Antibody (Mab9)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

4 Published Figures

17 References

View all (14) RAB9 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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RAB9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of RAB9 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB9 Mouse Monoclonal Antibody (Product # MA3-067) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HEK-293 cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HEK-293 cells showing cytoplasmic (Golgi, ER, endosome pattern) and plasma membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

RAB9 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing RAB9 (Product # MA3-067) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

RAB9 Antibody in Western Blot (WB) — Western blot was performed using Anti-RAB9 Mouse Monoclonal Antibody (Product # MA3-067) and a 23 kDa band corresponding to RAB9 was observed in cell lines tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2) and MCF7 (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

RAB9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with RAB9A siRNA and reduction of signal was observed in Western Blot using RAB9 Mouse Monoclonal Antibody (Product # MA3-067). {KD}

Product Details

MA3-067

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	1:20	View 5 publications 5 publications
Immunocytochemistry (ICC/IF)	1:200-1:500	View 6 publications 6 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-dependent	-
Immunomicroscopy (IM)	Assay-dependent	-

Product Specifications
Species Reactivity	Bovine, Dog, Hamster, Human, Non-human primate, Rat
Published species	Hamster, Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	Mab9
Immunogen	Recombinant canine rab 9a.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites diluted in PBS
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2175599

Product Specific Information

MA3-067 recognizes prenylated and non-prenylated rab 9 from canine, human, monkey, rat, hamster and bovine tissues. This antibody does not cross-react with other rab family members.

MA3-067 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~26 kDa protein representing rab 9 from K562 cell extract.

The MA3-067 antigen is recombinant canine rab 9a.

Target Information

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Herpes Simplex Virus Entry by a Nonconventional Endocytic Pathway.

Journal of virology

Tebaldi G,Pritchard SM,Nicola AV

MA3-067 was used in Western Blot to characterize Herpes simplex virus 1 entry by endocytosis.

Mon Nov 23 00:00:00 EST 2020

Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway.

Frontiers in cellular neuroscience

Lambert L,Dubayle D,Fafouri A,Herzog E,Csaba Z,Dournaud P,El Mestikawy S,Bernard V

MA3-067 was used in Immunocytochemistry to examine the dynamics of the muscarinic m2 receptor after agonist activation in living hippocampal neurons, and especially clathrin dependency endocytosis.

Tue Sep 29 00:00:00 EDT 2020

Endosomal trafficking defects in patient cells with KIAA1109 biallelic variants.

Genes & diseases

Kane MS,Diamonstein CJ,Hauser N,Deeken JF,Niederhuber JE,Vilboux T

MA3-067 was used in Immunocytochemistry-immunoflourescence to study the mechanism by which KIAA1109 loss of function causes this spectrum of congenital neurological malformation disorders.

Fri Mar 01 00:00:00 EST 2019

Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons.

The Journal of cell biology

Cheng XT,Xie YX,Zhou B,Huang N,Farfel-Becker T,Sheng ZH

MA3-067 was used in Immunohistochemistry to characterise LAMP1 distribution in autophageic and endolysosomal organelles in neurons.

Mon Sep 03 00:00:00 EDT 2018

Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function.

Journal of neurochemistry

Janickova H,Prado VF,Prado MAM,El Mestikawy S,Bernard V

MA3-067 was used in Immunohistochemistry to demonstrate that ChAT-ChR2 mice present major alterations of CIN neuronal morphology and increased behavioral sensitization to cocaine, supporting the notion that the increased levels of VAChT observed in these mice make them fundamentally different from wild-type mice.

Fri Sep 01 00:00:00 EDT 2017

BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.

The Journal of cell biology

Zhang X,Jiang S,Mitok KA,Li L,Attie AD,Martin TFJ

MA3-067 was used in Western Blotting to identify C2 domain-containing proteins involved in the regulation of Ca(2+)-dependent dense-core vesicle exocytosis in neuroendocrine cells.

Mon Jul 03 00:00:00 EDT 2017

Dynamic internalization and recycling of a metal ion transporter: Cu homeostasis and CTR1, the human Cu⁺ uptake system.

Journal of cell science

Clifford RJ,Maryon EB,Kaplan JH

MA3-067 was used in immunocytochemistry study how importation of copper into cells is regulated

Fri Apr 15 00:00:00 EDT 2016

Microvillus inclusion disease: loss of Myosin vb disrupts intracellular traffic and cell polarity.

Traffic (Copenhagen, Denmark)

Thoeni CE,Vogel GF,Tancevski I,Geley S,Lechner S,Pfaller K,Hess MW,Müller T,Janecke AR,Avitzur Y,Muise A,Cutz E,Huber LA

MA3-067 was used in immunohistochemistry and western blot to study the role of defective intracellular trafficking and loss of cell polarity due to myosin Vb loss in the pathogenic mechanism of microvillus inclusion disease

Wed Jan 01 00:00:00 EST 2014

High prevalence of retinoblastoma protein loss in triple-negative breast cancers and its association with a good prognosis in patients treated with adjuvant chemotherapy.

Annals of oncology : official journal of the European Society for Medical Oncology

Treré D,Brighenti E,Donati G,Ceccarelli C,Santini D,Taffurelli M,Montanaro L,Derenzini M

MA3-067 was used in immunohistochemistry to study the status of retinoblastoma protein in triple-negative breast cancers and its prognostic significance

Sun Nov 01 00:00:00 EDT 2009

Increased mucosal nitric oxide production in ulcerative colitis is mediated in part by the enteroglial-derived S100B protein.

Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society

Cirillo C,Sarnelli G,Esposito G,Grosso M,Petruzzelli R,Izzo P,Calì G,D'Armiento FP,Rocco A,Nardone G,Iuvone T,Steardo L,Cuomo R

MA3-067 was used in immunohistochemistry to study the mechanism for the increased production of mucosal nitric oxide in ulcerative colitis

Sun Nov 01 00:00:00 EDT 2009

Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain.

New biotechnology

Vernet E,Lundberg E,Friedman M,Rigamonti N,Klausing S,Nygren PA,Gräslund T

MA3-067 was used in flow cytometry to study the role of epidermal growth factor receptor in tumor cell proliferation

Tue Sep 01 00:00:00 EDT 2009

Coexpression of the melatonin receptor 1 and nestin in human breast cancer specimens.

Journal of pineal research

Rögelsperger O,Ekmekcioglu C,Jäger W,Klimpfinger M,Königsberg R,Krenbek D,Sellner F,Thalhammer T

MA3-067 was used in immunocytochemistry to study the expression and role of the melatonin receptor 1 and nestin in human breast cancer

Fri May 01 00:00:00 EDT 2009

Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication.

Journal of virology

Belov GA,Habbersett C,Franco D,Ehrenfeld E

MA3-067 was used in western blot to study the role of cellular Arf GTPases during poliovirus replication

Sat Sep 01 00:00:00 EDT 2007

Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.

Molecular biology of the cell

Ivanov AI,Nusrat A,Parkos CA

MA3-067 was used in immunocytochemistry to investigate the mechanism of endocytosis of adherens junction proteins and tight junction proteins in T84 epithelial cells

Thu Jan 01 00:00:00 EST 2004

Nitric oxide-dependent processing of heparan sulfate in recycling S-nitrosylated glypican-1 takes place in caveolin-1-containing endosomes.

The Journal of biological chemistry

Cheng F,Mani K,van den Born J,Ding K,Belting M,Fransson LA

MA3-067 was used in immunocytochemistry to investigate the processing of heparan sulfate side chains from recycling glypican-1

Fri Nov 15 00:00:00 EST 2002

Kaposi's sarcoma-associated herpesvirus K3 utilizes the ubiquitin-proteasome system in routing class major histocompatibility complexes to late endocytic compartments.

Journal of virology

Lorenzo ME,Jung JU,Ploegh HL

MA3-067 was used in western blot to study the function of Kaposi sarcoma-associated herpesvirus K3 in internalization and targeting of MHC class I complexes.

Sat Jun 01 00:00:00 EDT 2002

Quantitative analysis of the interactions between prenyl Rab9, GDP dissociation inhibitor-alpha, and guanine nucleotides.

The Journal of biological chemistry

Shapiro AD,Pfeffer SR

MA3-067 was used in western blot to investigate the interaction among prenyl Rab9, GDI and guanine nucleotides

Fri May 12 00:00:00 EDT 1995

Bioinformatics

Protein Aliases: rab-9A; rab9; rab9 GTP-binding protein; RAB9, member RAS oncogene family; RAB9-like protein; RAB9A, member RAS oncogene family; Ras-related protein Rab-9A

Gene Aliases: RAB9; RAB9A

UniProt ID: (Human) P51151, (Dog) P24408, (Rat) Q99P75

Entrez Gene ID: (Human) 9367, (Bovine) 511776, (Dog) 403947, (Rat) 84589, (Chinese hamster) 100767493

Function(s)

small GTPase mediated signal transduction protein transport regulation of protein localization retrograde transport, endosome to Golgi positive regulation of exocytosis negative regulation by host of symbiont molecular function Rab protein signal transduction intracellular protein transport metabolic process phagosome-lysosome fusion

Process(es)

GTPase activity protein binding GTP binding GDP binding identical protein binding