Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:5,000 | View 5 publications 5 publications |
Immunohistochemistry (IHC) |
1:20 | View 5 publications 5 publications |
Immunocytochemistry (ICC/IF) |
1:200-1:500 | View 6 publications 6 publications |
Flow Cytometry (Flow) |
- | View 1 publication 1 publication |
Immunoprecipitation (IP) |
Assay-dependent | - |
Immunomicroscopy (IM) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Bovine, Dog, Hamster, Human, Non-human primate, Rat |
Published species |
Hamster, Human, Mouse |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
Mab9 |
Immunogen |
Recombinant canine rab 9a. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
ascites diluted in PBS |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2175599 |
MA3-067 recognizes prenylated and non-prenylated rab 9 from canine, human, monkey, rat, hamster and bovine tissues. This antibody does not cross-react with other rab family members.
MA3-067 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~26 kDa protein representing rab 9 from K562 cell extract.
The MA3-067 antigen is recombinant canine rab 9a.
Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: rab-9A; rab9; rab9 GTP-binding protein; RAB9, member RAS oncogene family; RAB9-like protein; RAB9A, member RAS oncogene family; Ras-related protein Rab-9A
Gene Aliases: RAB9; RAB9A
UniProt ID: (Human) P51151, (Dog) P24408, (Rat) Q99P75
Entrez Gene ID: (Human) 9367, (Bovine) 511776, (Dog) 403947, (Rat) 84589, (Chinese hamster) 100767493
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