|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant, full length, human RAC protein.|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/ml|
|Immunoprecipitation (IP)||4 µg|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20580 detects RAC in human, mouse, and rat samples.
MA1-20580 has been successfully used in immunohistochemistry (frozen), immunoprecipitation, and Western blot procedures. Detects a band of approximately 21 kDa.
The MA1-20580 immunogen corresponds to recombinant, full length, human RAC protein.
Store at 4°C short term. For extended storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia and filopodia. It is involved in cytoskeletal actin organization and transformation. The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42 which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts. RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Casein kinase 2 phosphorylation of protein kinase C and casein kinase 2 substrate in neurons (PACSIN) 1 protein regulates neuronal spine formation.
MA1-20580 was used in immunocytochemistry and western blot to examine the involvement of PACSIN 1 in the BDNF-induced spine formation
|Schael S,Nüchel J,Müller S,Petermann P,Kormann J,Pérez-Otaño I,Martínez SM,Paulsson M,Plomann M||The Journal of biological chemistry (288:9303)||2013|
Induction of nonapoptotic cell death by activated Ras requires inverse regulation of Rac1 and Arf6.
MA1-20580 was used in blocking or activating experiment to investigate the role of Ras, Rac1 and Arf6 in methuosis
|Bhanot H,Young AM,Overmeyer JH,Maltese WA||Molecular cancer research : MCR (8:1358)||2010|
Nitric oxide and cyclic GMP regulate early events in agrin signaling in skeletal muscle cells.
MA1-20580 was used in western blot to study the involvement of nitric oxide and cyclic GMP in agrin signaling in skeletal muscle cells
|Godfrey EW,Schwarte RC||Experimental cell research (316:1935)||2010|