|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Amino acids 1-425 of Rad50 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-23269 detects Rad50 in human samples.
MA1-23269 has been successfully used in immunofluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures.
MA1-23269 immunogen is residues 1-425 of Rad50 expressed in E. coli..
Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Positional stability of single double-strand breaks in mammalian cells.
MA1-23269 was used in immunocytochemistry and western blot to study the mechanism for chromosome translocation in living mammalian cells
|Soutoglou E,Dorn JF,Sengupta K,Jasin M,Nussenzweig A,Ried T,Danuser G,Misteli T||Nature cell biology (9:675)||2007|